1% Agarose Gel DNA Electrophoresis

1
1 Agarose Gel DNA Electrophoresis
Running time 52 minutes Distance measured from
the well to the top of the second band 4,5
cm Running voltage 100 Volt Solidification
time 14 minutes Composition 250 mikroL buffer
TAE 2,5 gram Agarose 20 mikroL Ethidium
Bromide
1 Kb
100 Kb
Scale in Cm
Second Band
2
2 Agarose Gel DNA Electrophoresis
Running time 52 minutes Distance measured from
the well to the top of the second band 3.4
cm Total Running time 62 minutes Total distance
measured 4.5 cm Running voltage 100
Volt Solidification time 21 minutes Composition
500 mL buffer TAE 10 gram Agarose 40 mikroL
Ethidium Bromide
1 Kb
100 Kb
Scale in Cm
Second Band
3
3 Agarose Gel DNA Electrophoresis
Running time 52 minutes Distance measured from
the well to the top of the second band 2.9
cm Total Running time 68 minutes Total distance
measured 4.5 cm Running voltage 100
Volt Solidification time 17 minutes Composition
250 mikroL buffer TAE 7.5 gram Agarose 40
mikroL Ethidium Bromide
1 Kb
100 Kb
Scale in Cm
Second Band
4
Overall comparison
1
2
3
Cross sections of 3 different concentration
of DNA Agarose Gel are illuminated under UV
light
5
Overall Comparison
1
2
3
Increasing the concentration of Agarose gel
decreases the electrpphoretic mobility of
DNA Larger Fragments are better resolved in the
low agarose concentration while higher
concentration allow resolution of smaller DNAs
1 kb
1 kb
100 bp
100 bp
1 kb
100 bp
6
Comparison5 mikroL of 1 Kb DNA Ladder in 1,2
and 3 Agarose Gel Electrophoresis
1
2
3
Wells level
3,0 kb
2,0 kb
3,0 kb
2,0 kb
7
Comparison5 mikroL of 100 bp DNA Ladder in 1,2
and 3 Agarose Gel Electrophoresis
1
2
3
Wells level
1000 bp
500 bp
1000 bp
500 bp