Live food aquaculture training course

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Enrichment productsfor rotifers
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Enrichment of rotifers

• Essential fatty acids
• eicosapentaenoic acid (EPA 205n-3)
• docosahexaenoic acid (DHA 226n-3)
• Arachidonic acid (ARA 204n-6)
• Proteins and essential amino acids
• Vitamins

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Essential Fatty Acids

• Eicosapentaenoic acid (EPA 205n-3), improves
  survival
• Docosahexaenoic acid (DHA 226n-3)
• accumulating in the brain of larval fish,
  improves pigmentation of flat fish, improves
  growth and development.
• Arachidonic acid (ARA 204n-6)
• substrate for producing eicosanoids
• 160n-9 and 181n-9 promote growth

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Enrichment with HUFAs

• Rotifers incorporate HUFAs within a few hours
• Rotifers are non selective for uptake and non
  selective for catabolism so can be enriched with
  lipids on a short term protocol but there is a
  danger that they can have too high lipid content.

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Vitamin C Ascorbic acid

• Vitamin C can assist with stress tolerance and
  reduce deformity (opercular plate for bream)
• Rotifers fed
• bakers yeast attain 150 mg/g -1 DW
• Chlorella attain 1000 to 23000 mg/g-1 DW
  (depending on algal quality)
• Ascorbyl palmate is accumulated by the rotifer
  and converted to free AA. 5 AP w/w in emulsion
  is converted into AA concentration of 1700 mg/g-1
  DW
• AA incorporated in the rotifer remains at high
  levels even after 24 hours in storage

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Ascorbic Acid and rotifers
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Enrichment with Vit C

• Enrichment using ascorbyl palmitate. AP is
  converted by the rotifers into active Ascorbic
  Acid up to 1700 mg.g-1DW after 24 h enrichment
  when using a 5 AP (w/w) emulsion

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Proteins

• Protein content of rotifers vary between 28 and
  67 of dry weight.
• Variability is dependant on nutritional status
• Proteinlipid ratio is positively correlated to
  SGR with fast growing rotifers (high protein to
  lipid ratios) giving better larval growth and
  survival (Turbot).
• Protein and has great affect on early larval
  production success.

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Phospolipids

• used for the formation of cell components
  particularly during rapid growth periods
• during rapid growth phospolipids probably not
  produced fast enough to meet requirements
• found to reduce malformation in larvae

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Algae

• Nannochloropsis rich in EPA and ARA
• Isochrysis rich in DHA
• Rotifers enriched with Isochrysis will attain a
  DHAEPA ratio of 2 within a few hours.

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Composition (INVE Products)
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Composition (RICH Products)
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Comparison Selco RICH
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Comparison of Algae Rich and CS3000
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Enrichment of rotifers with RICH
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Comparison RICH Advanced, Top S.S.