DNA Extraction

1
DNA Extraction

• Common molecular biology lab procedure
• Why extract DNA ?
• To study DNA structure and function
• Paternity testing/Genetic testing
• Forensics

2
Key Players

• Cells DNA stored in nucleus
• Salt (NaCl)
• DNA has a negative charge
• Na neutralizes DNAs charge

3
Key Players

• Detergent
• dissolves lipids of membrane

4
Remove Proteins

• Enzyme added
• Protease breaks down the proteins
• cuts histones from DNA

http//learn.genetics.utah.edu/units/activities/ex
traction/enzyme
5
Precipitate DNA

• Ethanol added
• DNA is insoluble in ethanol (clumps together)

DNA
ethanol
cells/soap/salt
http//learn.genetics.utah.edu/units/activities/ex
traction