Biotechnology: Presant Status

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Biotechnology Presant Status biosafety
concernsDepartment of Agriculture

• H. M.J.Bandara
• Plant Quarantine Officer
• Sri Lanka

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Mandatory CropsCereals, legumes, condiments,
fruit crops, root tuber crops, oil seed crops
vegetables, floricultural plants
Department of Agriculture

• Activities
• Research development, Seed certification
  plant protection, Planting material production
  Distribution Plant quarantine, Germplasm
  management, Botanic gardens, Agriculture
  extension, Education training,
• Finance administration

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Tissue CultureMolecular techniques (Based on
nuclear acid proteins)
Biotechnology
Biotechnology is defined in many ways. Tissue
culture is considered one of the techniques.
Its associated directly with genetic
transformation.


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HORTICULTURAL CROP RESEARCH AND DEVELOPMENT
INSTITUTE- Tissue Culture Program

• Banana Tissue Culture
• Papaya tissue culture

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HORTICULTURE CROP RESEARCH AND DVELOPMENT
INSTITUTE

• Virus indexing (serological)
• Banana
• Papaya
• Citrus
• Microbiology
• Food Research Unit
• Fermentation Food analysis

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PLANT VIRUS INDEXING CENTER

• Serology and PCR Based Virus Indexing- Banana
  Virus
• Pineapple
• RT PCR for Detection of RNA Virus

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SITHA ELIYA POTATO RESEARCH CENTERTissue
Culture Program Potato

• Straw berry

Pineapple
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RARDC, Bandarawela

• Tissue Culture Program
• Potato
• Anthurium
• Gerbera
• Strawberry
• Madonna lily
• Virus indexing of potato by ELISA

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RARDC, Agunakolapelessa

• Banana
  

• Thubakarwial

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RICE RESEARCH AND DEVELOPMENT INSTITUTERice
Biotechnology

• Anther Culture
• Exploitation of Somaclonal Variation
• Wide Hybridization

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NATIONAL BOTANIC GARDENS-Tissue Culture Program

• Propergation of Endemic rare Plants
• Floriculture Plants
• Mutation induction

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PLANT GENETIC RESOURCE CENTER Tissue Culture
Program

• Banana
• Citrus spp

• In vitro pineapple potato Conservation
• Papaya tissue Culture

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PLANT GENETIC RESOURCE CENTER Tissue Culture
Program

• Anther Culture in rice Haploid Culture
• a good system for transformation

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PGR/RRDI- Rice researchPCR based DNA markers
for breeding/gene pyramiding

• Gall midge resistance

• Thrips resistance

• Bactria leaf blight resistance

• BPH resistance

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Genetic Characterization by DNA markers

• Mango - Mangifera zeylanica Magifera indica
• Maize(PGRC/MI)
• Finger printing of hybrids
• Pathogens - Ralstonia, BLB
• Onion - Characterization breeding

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Wild tomato X tomato
Introgression of genes from wild relatives

• Wild okra X okra

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Future Work

• SSR markers for rice
• AFLP for fingerprinting of germplasm Varieties
• Northern blot techniques for
• Analysis of HSP regular gene Expression
• Isolating the fragment for Coat protection gene
  papaya Ring spot virus

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Application of Tools in Biosafety Regular Measures

• Tissue Culture Safe handling of materials
  Restricted multiplication
• Serological methods Testing detection of
  foreign
• Genes bases in proteins
• Serological method should not be the sole
  method.
• Genes expression (transgenic)is spatially
  temporally controlled
• PCR/Blot methods Based on DNA More sensitive
  method.Detect only presence or foreign DNA
  

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National Plant Quarantine Service

• Requirements
• With the existing infrastructure function and
  provided with additional strength (Trained staff,
  Detection equipment, Risk assessments Studuo)
  NPQS could undertake the regulatory activity
  

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Institutional Biosafety Committee

• Liaison with the national committees
• Identification of institutestransformation
  work,GMO detection /testing,regulation risk
  assessment
• Preparation of guidelines on biosafety
  regulations,risk assessments management
• Maintain constant access to relevant data base or
  registers both local foreign
• Monitoring evaluation of programs
• Building awareness

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Requirements for Implementation of the Biosafety
Procedures

• Policy level support
• Financial,administrative legislative support
• Biosafety clearing house mechanism
• Monitoring, evaluation inspection services
• Training of decision makers,technical level
  scientists technicians
• Facility enhancement
• Public awareness, education promotion of public
  participation including stake holders.

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GENETIC Transformation ofAgricultural Crops

• Policy level decisions if necessary, changes
• Identification of priority problems/crops
• Identification of Institutes
• Facility development

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THANK YOU