Lecture 15: DNA Organization, Replication and Repair

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Lecture 15 DNA Organization, Replication and
Repair   Reading Assignment Chapter 38, pgs.
412-434 Harpers Biochemistry (25th
edition).   Objective To understand the
structures and proteins involved in DNA
replication.     I. Cellular DNA exists as
chromatin   A. Nucleosomes   1. Histones   II.
General characteristics of DNA replication   A.
Semi-conservative   B. Starts at specific
sites   C. Bidirectional   III. General
characteristics of DNA polymerases   A. Use DNA
as a template   B. Replicate DNA with high
fidelity   C. Catalyze the formation of
phosphodiester bridge   IV. Characteristics and
function of procaryotic DNA polymerases   V.
Characteristics and function of eucaryotic DNA
polymerases  
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VI. Characteristics and function of other
proteins involved in DNA replication   VII. Steps
in DNA replication   VIII. DNA replication at
chromosome ends   A. General structure of
chromosome ends   B. Enzyme involved in
replication of chromosome ends   IX. Types of
DNA mutations   A. Single base
alterations   1. Transitions   2.
Transversions   B. Two base alterations 1.
Pyrimidine dimers   C. Chain breaks   D.
Cross links   X. Types of DNA repair
systems   A. Direct repair   B. Excision
repair   C. Mismatch repair
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