But is it art

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But is it art?

• Brandeis University
• 12.07.05

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Will the real artist please stand up?
and their art
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(No Transcript)
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Genetically-encoded Oscillator
Period is 120 (two doublings)
Time (minutes)
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Electronics 101
Hartley Oscillator
Colpitts Oscillator
http//my.integritynet.com.au/purdic/oscillators.h
tm
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1. Motivation why engineer biology?
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1. Motivation why engineer biology? 2.
Plan how to engineer biology?
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1. Motivation why engineer biology? 2.
Plan how to engineer biology? 3.
Execution state of the art (stupid bacteria
tricks)
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1. Motivation why engineer biology? 2.
Plan 3. Execution
because we can because theres so much to
build because theres so much to learn
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Genetically-Encoded Digital Logic
transfer function
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Heat Shock Response s32 induction
Straus, Walter, and Gross Nature (1987) 329 347
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Genetically-Encoded Logic Functions
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Genetically-Encoded Logic Functions
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Genetically-Encoded Logic Functions
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Genetically-Encoded Logic Functions
X
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Logic Functions
Inverter
AND gate
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Programming with DNA hiding the details
Genetic Inverter
repressor gene
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Programming with DNA hiding the details
Genetic Inverter
repressor gene
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Programming with DNA hiding the details
Genetic Inverter
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Synthetic Biology programming with DNA
Nature is source for code Genetic Parts
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There is a great satisfaction in building good
tools for other people to use. -Freeman Dyson
Biology
Engineering
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There is a great satisfaction in building good
tools for other people to use. -Freeman Dyson
Biology
Engineering
how can we help?
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There is a great satisfaction in building good
tools for other people to use. -Freeman Dyson
Biology
Engineering
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1. Motivation why engineer biology? 2.
Plan 3. Execution
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1. Motivation 2. Plan how to engineer
biology? 3. Execution
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1. Motivation 2. Plan how to engineer
biology? 3. Execution
what foundational technologies are needed? are
safeguards needed?
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Works of Art
A good scientist is a person with original
ideas. A good engineer is a person who makes a
design that works with as few original ideas as
possible. There are no prima donnas in
engineering. -Freeman Dyson
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Attributes for Foundational Technologies
well characterized predictable modular insulated s
table
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Attributes for Foundational Technologies
well characterized predictable modular. insulated
stable
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http//parts.mit.edu
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Standard Assembly
http//parts.mit.edu
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Broadly consider two sources of risk 1. from
the technology itself 2. from the people
(hackers) who use it
G. Church, Let us go forth and safely multiply
Nature (2005) 438423
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1. Motivation 2. Plan how to engineer
biology? 3. Execution
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1. Motivation 2. Plan 3. Execution state of
the art
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1. Motivation 2. Plan 3. Execution state of
the art
SB accomplishments undergrad. contributions
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(No Transcript)
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Nature (2005) 434 1130
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A new kind of biofilm coli-roid
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Light Dependent Gene Expression
Phytochromes from Synechocystis
Osmoregulation system from E. coli
LacZ reporter strain from literature (1979)
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Light Dependent Gene Expression
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Biofilm v2.0
mRFP
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1. Motivation why engineer biology? 2.
Plan how to engineer biology? 3.
Execution state of the art
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Three comic strips to conclude
Worst case scenario total failure
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Three comic strips to conclude
Worst case scenario total failure Best case
total success, are we ready?
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Three comic strips to conclude
Worst case scenario total failure Best case
total success, are we ready? Biological
Engineers, an oxymoron?
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Credits
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the end
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(No Transcript)