First experiment

1
First experiment

• TE has negative effect on expression

2
03g10620 1kb promoter with LINE room temperature
no expression
03g10620 without LINE 527bp room temperature
expressed
03g10620 with LINE 1kb room temperature not
expressed
03g10620 527bp without LINE promoter salt stress
expressed in root and stem
03g10620 with LINE1kb promoter salt stressed no
expression
3
With retro, room tempt
4
Without retro, room tempt
5
1.1 With retro, room tempt
6
1.2 With retro, room tempt
7
2. Without retro, room tempt
8
2nd experiment
TE has negative effect (down-regulation) on
expression and the promoter lost function in
salt stress
9
03g10620 1kb promoter with LINE room temperature
almost no GUS expression
03g10620 with LINE1kb promoter salt stressed no
expression
03g10620 527bp without LINE promoter salt stress
almost no expression
03g10620 without LINE 527bp promoter room
temperature GUS expressed
10
By Surendars camera
11
03g10620 with LINE1kb promoter salt stressed no
expression
03g10620 1kb promoter with LINE room temperature
almost no GUS expression
03g10620 527bp without LINE promoter salt stress
almost no expression
03g10620 without LINE 527bp promoter room
temperature GUS expressed
12
Also tried unscratched rice
With LINE room tempt
With LINE salt stress
Without LINE room tempt
Without LINE salt stress
13
Natural genes expression driven by promoter
03g10620
TE is present in 93-11.