YinYang Column for Global Protein Identification

1
Yin-Yang Column for Global Protein Identification

• Column Technology Inc.

2
The Yin-Yang column combines anion and cation
exchange chromatography For the protein
identification through multi-LC/MS/MS
Yin-Yang MDLC-MS/MS
3
Advantages of Yin-yang MDLC-MS/MS

• Three-dimensional LC-MS/MS
• SCX and ANX are complimentary methods for peptide
  fractionation
• Most complete coverage of peptides and proteins
• Enrichment and high-sensitivity for
  phosphopeptides
• Identify phospho and non-phospho peptides in the
  same run.

4
Theoretical pI distribution of identified
non-phosphrylated peptides
Complementary peptide identification of SCX and
SAX
5
108413 Unique peptides
SAX
SCX
3047
9094
10254
6184 Proteins
SCX
SAX
2040
4152
4072
only 1mg protein digests of mouse liver, 5
FPR Another More than 1000 phosphopeptides
identified