Chlamydomonas reinhardtii

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Chlamydomonasreinhardtii
10um
(They) provide the divers incredibly thin feet .
. . Which were moved very nimbly - Van
Leeuwenhoek, 1675
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EM
Mitochondria
Chloroplast
Basal Bodies
Pyrenoid
Nucleus
Nucleolus
Wall
Golgi
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The Chlamydomonas Genomes

• Nuclear genome 17 chromosomes 120,000 Kbp
• encoding 15,256 ORFs
• Chloroplast genome 1 circular chromosome,
  203,395 bp
• Mitochondrial genome 1 circular chromosome,
  15,800 bp

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Human Flagella and Cilia
Normal patient
primary ciliary diskinesia patient
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Photoreceptors are Modified Cilia
Rod cell Cone cell
Modified flagellum
Modified flagellum
Basal Body
Synapses
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Bio-Logical Answers
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The Chlamydomonas Microtubule Cytoskeleton
Cytoplasmic microtubuules Flagellar axonemes
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Chlamy Mitosis
Microtubules DNA
Overlay
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Chlamy Life Cycle
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Chlamy Mating
Actin-containing mating structure Flagella
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The Eukaryotic Flagellum
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Basal Bodies
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Microtubule Sliding Powers Flagellar Beating
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The Flagellar Dynein Motor Is (a) Complex
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Axonemal Abnormalities
Primary Ciliary Diskinesia
Normal Kartageners syndrome
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Keeping the Beat!
Disoriented cilia in a PCD patient
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Focus Flagellar Assembly
TZ BB
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Dikaryon ExperimentsTubulin
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Dikaryon RescueRadial Spoke Proteins
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Building the FlagellumIntraFlagellar Transport
(IFT)
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A Few Questions for Thought

• Compare and contrast the cell biology of
  Saccharomyces and Chlamydomonas
• Compare and contrast the life cycles of
  Saccharomyces and Chlamydomonas
• Given that we know the complete genomic sequences
  of both of these eukaryotic cells, discuss what
  might be gained by comparing the genomes,
  transcriptomes and proteomes of these model
  organisms
• Review your notes from the first semester on
  MTOCs/centrosomes/centrioles and flagella and
  extend your knowledge of the structure, function
  and assembly of the eukaryotic structures
• Compare and contrast prokaryotic and eukaryotic
  flagella