Splice-MO

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Uninjected 24-28 hpf Prim 5-6
Splice-MO Injected 24-28 hpf
86 Survival 7 Normal
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Muscular Twitching 21-somite embryos
Uninjected 21-Somites
2-4 ng Splice-MO Injected
86 Survival 7 Normal
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Xp120ctn Rescues Migration Defects
Red LineYolk Extension Length Green LineTail
Length
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Active or phosphorylated focal adhesion kinase
concentrates at organ boarders with
extracellular matrix
8-10 Somites 24 hpf
pY397Fak
Hille lab- Crawford et al, 2003
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Xp120ctn Rescues Migration Defects
Uninjected
4ng Sp-MO 50pg Xp120 mRNA
50pg Xp120 mRNA
4ng Sp-MO
15hpf14 Somites
Ryan Walsh Cynthia Hsu My Nguyen
17hpf17 Somites
30hpf prim-15
Red LineYolk Extension Length Green LineTail
Length
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Possible Implications

• All vertebrates have the same or very similar
  mechanism for this movement of cell.
• Zebrafish can be used as a model for the study of
  humans cells movement.
• Understanding of birth defects

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p120 Activates Cell Motility



p120 ctn
Anastasiadis, 2007
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How do cells move?
adhesion proteins
regulator proteins
actin stimulation proteins
actin proteins
matrix
Cell-cell adhesion
Cell-matrix adhesion
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adherens junctions
desmosomes
tight junctions
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How do cells move? Protein regulate movements
adhesion proteins
regulator proteins
actin stimulation protein
actin proteins
matrix
Cell-cell adhesion
Cell-matrix adhesion
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p120 Activates Cadherin-Mediated Adhesion
Anastasiadis, 2007