A novel probe for localized manganese delivery

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A novel probe for localized manganese
delivery Christian Brekken Marte
Thuen Trond E. Singstad Olav Haraldseth
Norwegian Molecular Imaging Center, NTNU
Trondheim, Norway. Axel Sandvig
Experimental Medical Research, Ullevål University
Hospital, Oslo, Norway. Martin Berry
Centre for Neuroscience, Kings College, London,
UK Yrr Mørch Berit L.
Strand Gudmund Skjåk Bræk The
Bioencapsulation Group, NTNU Trondheim, Norway.
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Alginate Composition
MGGGGGGMMMMMMMMGMGMGMGMMMMMM
G-block
M-block
MG-block
M-block
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Alginate sources and composition

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Binding / Ion Exchange
Affinity Mn lt Zn, Ni, Co lt Ca lt Sr lt Ba lt Cd lt
Cu lt Pb
Flexibilty GG lt MM lt MG
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Calcium Alginate / Eggbox Model
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Manganese Alginate / Eggbox Model
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Manganese-Alginate gels
400 mm
400 mm
400 mm
1M MnCl2
1M MnCl21mM BaCl2
50mM CaCl2, 1M MnCl2
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MRI results - in vitro
Incubation solution 1M MnCl2 1mM BaCl2
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MRI results - in vitro
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MRI results - in vivo
Animal models
Rats
Fish
Frogs
Mice
CNS regeneration after damage 10 100
100 10
Comparative physiology in search for functional
genes (transgenic animals)
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MRI results - in vivo
MRI 2.35T BIOSPEC, 3D FLASH, TE/TR/FA4.2ms/15ms/
25deg, Voxel size165x234x234mm3, TET65min
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MRI results - in vivo
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Mn-alginate design - what can we play with?

• Alginate core composition (G-content)
• Alginate cross binding ion(s)
• Alginate sphere size
• Injection preparation (wet/dry)
• Number of alginate spheres injected

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Mn-alginate design - what can we play with?

• Alginate core composition (G-content)
• Alginate cross binding ion(s)
• Alginate sphere size
• Injection preparation (wet/dry)
• Number of alginate spheres injected

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Mn-alginate design - what can we play with?

• Alginate core composition (G-content)
• Alginate cross binding ion(s)
• Alginate sphere size
• Injection preparation (wet/dry)
• Number of alginate spheres injected

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Mn-alginate design - what can we play with?

• Alginate core composition (G-content)
• Alginate cross binding ion(s)
• Alginate sphere size
• Injection preparation (wet/dry)
• Number of alginate spheres injected

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Mn-alginate design - what can we play with?

• Alginate core composition (G-content)
• Alginate cross binding ion(s)
• Alginate sphere size
• Injection preparation (wet/dry)
• Number of alginate spheres injected

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Mn-alginate design - what can we play with?

• Alginate core composition (G-content)
• Alginate cross binding ion(s)
• Alginate sphere size
• Injection preparation (wet/dry)
• Number of alginate spheres injected

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Mn-alginate design - what can we play with?

• Alginate core composition (G-content)
• Alginate cross binding ion(s)
• Alginate sphere size
• Injection preparation (wet/dry)
• Number of alginate spheres injected

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Mn-alginate design - what can we play with?

• Alginate core composition (G-content)
• Alginate cross binding ion(s)
• Alginate sphere size
• Injection preparation (wet/dry)
• Number of alginate spheres injected

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Mn-alginate design - what can we play with?

• Alginate core composition (G-content)
• Alginate cross binding ion(s)
• Alginate sphere size
• Injection preparation (wet/dry)
• Number of alginate spheres injected