Laboratory of Molecular Technology LMT

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Laboratory of Molecular Technology (LMT)
915 Tollhouse Road, Frederick, MD
http//web.ncifcrf.gov/rtp/labs/LMT/
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• Complete genomics facility
• Technology platforms
• Genomic analysis
• Molecular interactions
• Gene expression
• Transcription
• Post-transcription
• Translation
• Post translation

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• Access and develop new technologies
• Availability to NCI researchers
• Core services
• Collaborations
• Apply to independent research programs
• Ovarian development
• Ovarian cancer
• Prostate cancer

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LMT Core Services

• Sequencing
• standard
• high-throughput
• Microarray
• Genotyping
• Custom automation
• Oligonucleotide synthesis
• Molecular diagnostics

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LMT Sequencing Core
4 ABI 377 slab-gel sequencers
3 ABI 3700 capillary sequencers

• Sequencing in tube, 96-well, or 384-well formats
• Data analysis

Current output 25,000 sequences / month (95
of theoretical capacity)
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GEOSPIZA INC.Software for Molecular Biology
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LMT Microarray Core
2 Axon GenePix 4000A microarray scanners
2 BioRobotics MicroGrid II microarray spotting
robots

• 400 Incyte GEM II mouse arrays distributed /
  month
• other libraries
• assistance with study design
• assistance with data analysis
• Affymetrix Workstation

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LMT Molecular Diagnostics Core

• CLIA certified
• Viral detection
• DNA quantitation
• Mouse speciation
• Mycoplasma detection
• Mutation detection

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LMT Oligonucleotide Synthesis Core

• Instrumentation
• 2 ABI 394 oligonucleotide synthesizers
• 1 ABI Ezpedite oligonucleotide synthesizer
• 1 HP 1015 HPLC
• Services offered
• standard oligonucleotides
• modified oligonucleotides
• PNA oligonucleotides
• HPLC purification
• Gel-purification
• Output 3500 oligonucleotides/month

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Other LMT Core Services

• Genotyping
• coming soon (July 2002)
• Custom automation
• automated colony picking
• re-array
• high density filter arrays (colony and DNA)
• automated process development
• custom automation software development

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LMT Research and Development

• Currently ongoing RD at LMT (independent and
  collaborative)
• expression profiling
• ovarian metastasis
• mouse ovarian development
• rat ovarian development
• mouse model of ovarian cancer
• p27 and prostate tumor regression
• prostate tumor prognostics and ethnicity
• caveolin 1 function during colon cancer
  development
• immune response

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LMT Research and Development

• Currently ongoing RD at LMT (independent and
  collaborative)
• microarray-based monoclonal antibody screening
  and selection
• sera microarrays
• DNA methylation / gene expression (NCI 60
  cell-line panel)
• electronic microarray development (Nanogen
  Corporation)
• high-throughput monoclonal antibody production
• multiplex analysis
• electronic stringency
• electronic addressing
• universal detection
• primer picking software

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LMT Research and Development

• Currently ongoing RD at LMT (independent and
  collaborative)
• functional SNP association and prostate tumor
  prognostics/ethnicity
• automated molecular screening
• high-throughput LOH analysis
• automated process development (Beckman Coulter)
• automated, high-throughput ABI 7900 genotyping
• universal detection system for ABI 7900
  genotyping
• virtual 2-D gel software
• real-time PCR primer picking software
• ABI 7900 genotyping primer picking software
• haplotype generation software

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LMT Research and Development

• RD at LMT (in development or planned)
• whole genome yeast 2-hybrid system
• DNA methylation changes during mouse ovarian
  development
• prostate tumor gene expressionLOH events

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• Collaborators
• NCI
• J. Weinstein
• M. Nickerson
• R. Bedigian
• J. Sharp
• J. Coffin
• S. OBrien
• M. Dean
• B. Gold
• J. Slattery
• Johns Hopkins Medical Center
• A. Feinberg
• University of Chile
• J. Allende
• A. Quest
• C. Cortes
• LSU Medical Center
• W. Rayford

LMT Group Leaders Microarray-Lisa
Gangi Genomics-Shirley Tsang Diagnostics-Kris
Pike Oligonucleotide synthesis- Leo
Lee Sequencing and Automation-Lynn
Rasmussen High-throughput-Claudia
Stewart Standard-John Elser Automation-Casey
Frankenberger