Biochemistry I

1
Biochemistry I

• Labs 22,23,24

2
Exoenzymes Lab 22

• Bacteria make enzymes to digest macromolecules
  outside of the cell
• The smaller sub units of the enzymatic reaction
  are then able to be transported across the cell
  membrane
• This capacity is a characteristic of many Gram
  Positive bacteria

3
Hydrolysis of starch

• Starch is composed of glucose molecules connected
  by glycosidic linkages
• When the glycosidic linkages are broken,
  individual sugar molecules are produced
• Linkages are broken in the presence of Water and
  the enzyme amylase

4
Hydrolysis of Gelatin

• When water and an enzyme are added to a protein,
  the peptide bond in proteins are broken,
  producing individual amino acids

5
Gelatin Hydrolysis in the tube
6
Carbohydrates and Sugars
7
Glycolysis
8
Sugar fermentation- Carbohydrate metabolism
9
Fermentation

• Phenol red pH indicator is red in a neutral
  environment and yellow is the result of an acidic
  pH
• Gas Gas is a by -product of the fermentation
  reaction -

10
www.jlindquist.net/generalmicro/dfnewgfbpage.html
11
Triple Sugar Iron

• a. metabolize lactose and/or sucroseb. conduct
  fermentation to produce acidc. produce gas
  during fermentationd. generate H2S.

12
TSI Results
13
Results
14
(No Transcript)