Molecular

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Molecular Workbenches Protein
structure function
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Lets make glutamine from glutamic acid
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What are the energy requirements? How do we
ensure specificity?
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DG DH - TDS Gfree energy Henthalpy Sentropy T
temperature
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Enthalpy is the energy content of a molecule
DHS bondsproducts-S bondsreactants
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Entropy is a measure of disorder
DSS disorderproducts -S disorderreactants
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The cell makes unfavorable reactions proceed
by coupling them to favorable reactions
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Two modes of coupling Direct coupling Sequentia
l coupling
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Sequential Coupling
AB ? CD D ? EF
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Amino Acids
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BREAK TIME
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Four levels of protein Structure
1-aa sequence 2-local folds/structure 3-structu
re of polypeptide 4-polypeptide interactions
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Two common folds a-helix b-sheet
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http//www.bio.cmu.edu/Courses/BiochemMols/ProtG/P
rotGImage.htm
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cytochrome b
antibody
NAD-binding
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Next Class How we study proteins