Prof' Dr' A' Hameed

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Quaid-i-Azam University Islamabad

• Prof. Dr. A. Hameed

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MICROBIAL PRODUCTION OF LYSINE FOR ITS USE IN
POULTRY FEED
University Industry
Department of Microbiology Quaid-I-Azam
University Islamabad
Barlas Poultry Rawalpindi
HIGHER EDUCATION COMMISSIONUniversity-Industry
Technical Support Program
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INTRODUCTION
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• Amino acids a-substituted carboxylic acids,
  building blocks for biomass constituents
  (proteins)
• Essential for
• Cell and body growth
• bone formation
• catalyses enzymatic reactions
• Sources
• Meat milk
• cereals, fruits vegetables

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• Asparagine was the first amino acid discovered in
  1806
• Out of main 20 types, at least 10 are not
  synthesized by the body and required to be
  included in food or feed (essential
  non-essential)
• Until 1957 amino acids were available exclusively
  by extraction methods or chemical synthesis
• In 1957, Kinoshita discovered Corynebacterium is
  a potential amino acid producer bacterium

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• In 1960, Kyowa-Hakko started MSG production by C.
  glutamicum
• More than 2 million tons Amino Acids are produced
  per year using coryneform bacteria
• Recent biological production of Lysine is 600,000
  tons with a growth rate of 7 per year
• Amino acids utilization in food, feed and
  pharmaceutical industry is (50,20 30)
• Main producers are Ajinomoto, Kyowa Hakko , ADM,
  Cheil Jedang, BASF and Degussa

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Market, Use and Production Method of Some Amino
Acids
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RESEARCH AT QAU

• At Microbiology Research Laboratory, QAU,
  Islamabad, microbial culture for production of
  Lysine has been established
• Different mutants of C. glutamicum were developed
• All the physical chemical parameters have been
  optimized for the growth production of Lysine
  from a mutant strain AHS-10 by shake flask
  fermentation

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• Stirred Tank Fermenter was used for process scale
  up experiments
• Nutrients, DOT, pH, Temp, Harvest time, agitation
  were monitored with respect to the production
  scale (1-6 L)
• 3L batch culture production for field trials
• Qualitative Quantitative analysis of Lysine in
  batch cultures

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FIELD TRIALS

• Feed formulation supplemented with different of
  produced Lysine
• Comparative efficacy of produced Lysine
  supplemented feed with respect to commercially
  available feed
• Field trials on commercial indigenous poultry
• Based on the results recommendations to be made
  for the microbial production of Lysine

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WEAKNESSES

• Improvements in industry-university liaison
  procedures
• Lack of trust of industrial management on
  national experts and products
• Conservative approach of industrialists to
  process change
• Intellectual Property Rights
• Lack of pilot plant Bioprocess facilities

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• Non availability of technical man power
• Lack of service and maintenance facility for
  equipments Service structure for researchers in
  Universities
• Service structure of supporting staff at
  universities
• Performance based selection/promotion/incentive
  (Tenure Track System)

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THANKS

• To HEC for initiating UITS Program
• To HEC Barlas Poultry Rawalpindi for providing
  funds for this project
• Prof. Altaf G. Sheikh CSF

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Microbiology Research Laboratory
Quaid-I-Azam University Islamabad