How To Grow Oyster Mushroom at Home?

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How To Grow Oyster Mushroom at Home?

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Title: How To Grow Oyster Mushroom at Home?


1
How to Grow Oyster Mushroom
2
Growing Oyster Mushrooms
  • All the members of oyster mushrooms are
    saprotrophic (feeds on wood). They are the best
    agents of mycorestoration and has also been found
    effective in various cancerous treatments.
    Moreover they make a delicious meal!
  • The medium of growth (substrate)
  • After intensive research wheat straw or cotton
    seeds are found to be two good medium though
    other substrates are also applicable.

3
Step1 Preparation of the medium (or substrate)
on which the mushroom grows
  • Materials required for creating such mediums are
    containers, plastic bags, liners, drums, heaters
    /burners, gloves, aprons, face masks.
  • Straw lengths of 5-10 cm should be immersed in
    water for 24 hours.
  • The batch should be properly washed before the
    straw is sent for pasteurization (the process of
    heating and then cooling).
  • A drum is filled with more than 30 litres of
    water and this is heated on a burner.
  • The bags of straw are placed in the drum and
    separated from water by a suitable platform
  • The drum is heated for 1 hr. till the time
    substantial steam flows through the straw bags
  • The straw bags are allowed to cool and the bags
    are removed to the clean growing room
  • At every stage the straw is checked for
    tenderness and it is made dirt free
  • Once the straw is prepared it is transferred to
    the growing room.

4
Step 2The Culture or Growing Room
  • The growing room is washed, sterilized with steam
    and bleached so that it is properly disinfected.
    The growing room should have ventilations for air
    flow and enough moisture to let the culture set
    in or the cells germinate. Temperatures of 10-24
    degree centigrade is usually maintained. Induce
    HEPA air filters to remove air impurities and
    keep a UV lamp.

5
Step 3 The Mushroom Spawn laboratory
  • The mushroom spawns are substrates (grain, agar
    cultures or liquid medium for inoculation) used
    to transfer the vegetative fungal growth or
    mycelia threads to a medium on which the mushroom
    grows.
  • Creating pure culture
  • 1. A small slice or tissue of mushroom is cut and
    added on PDA medium. In a few days hyphae
    (mycelium) covers the entire plate.
  • 2. Generation of mother spawn
  • 3. Propagation
  • Preparation of mother spawn
  • A small slice of mycelium with the PDA is taken
    from pure culture, induced in fresh medium and
    then supplements and grains added. Incubation for
    15-20 days at specific temperatures creates the
    mother spawn.
  • Propagation
  • The mother spawn is then allowed to propagate
    before usage

6
Step 4 Inoculating the Bags
  • The bags of straws should be opened and teaspoons
    of spawns should be added. Proper precautions
    regarding sterilization and hygiene must be
    maintained. Masks and lab uniform must be worn
    before inoculation

7
Step 5 Monitoring the Bags
  • No insect mice or pest infestation should be
    there. Moreover the bags should be regularly
    checked to ensure that harmful or non-productive
    strains does not grow.

8
Step 6 Incubation and Colonialisation
  • At proper humidity and with right amount of
    spawn, the mycelia takes three weeks to run
    through the whole straw. More amount of straw
    aids the process but runs risk of degeneration.

9
Step 7 CO2 Removal
Take care to flush the room with clean air during
the entire process so that CO2 removal happens,
otherwise deformities can be found in the
culture.
10
Step 8 Harvesting
  • As the bags get completely colonized with the
    mycelia, fruiting or pinning may be seen. At this
    stage, we need to remove the substrate and
    transfer the fruiting to a high humid, clean
    room. With fruiting, the humidity must be
    increased. Once the mushroom has grown, stop
    water sprays, you will observe the mushroom
    turning into yellowish brown color. Allow the
    entire batch of mushrooms to grow and acquire the
    desired color.

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Step 9 Removing the mushrooms from the culture
or growing room
  • Once the yellowish color is attained carefully
    pick the better quality mushrooms from the
    substrate. It should be taken care that the
    mushrooms are not washed before packing. Retain
    the mushrooms or pack them in damp (not wet)
    brown papers. Store them at room temperature
    before packing.

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Step 10 Packaging
  • The mushrooms are set on a vibrating tray to
    remove the impurities or other elements. Then are
    then dropped into quality chamber and after
    screening of the batches for unwanted batches,
    they roll into small perforated plastic bags (to
    allow enough air) and the bags are heat pressed
    at the seal. Labelling is done after that. They
    may even be canned or used for other purposes.
  • Then the entire batches after screening are
    delivered through channels before it reaches your
    plate as a delicious recipe.

13
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