Safety in a Bioengineering Lab

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Safety in a Bioengineering Lab
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Safety rule 1
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Sure, you are safe.

• Even if you think you are not going to have a
  problem
• (and you dont need goggles, etc)..
• Safety rule 2 Be cautious and vigilant
• the person next to you may not be following
  safety rules

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Safety Rule 3 Dress for the lab
No!
Yes!
Hair pulled back
Sleeves can be rolled up
Long pants in sturdy fabric
Closed toe shoes
Minimal jewelry
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Dressed for the lab on runways
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Safety Rule 4 Chemicals can be hazardous!

• Know the hazards of chemicals you use
• Causticburns the skin or eyes
• Volatileevaporates and fumes can be inhaled
• Flammablecan catch on fire easily
• Toxic or carcinogeniccan cause illness or death
  in short term or long term
• Listen to your instructor
• READ LABELS and remain focused

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Safety Rule 5- Be careful around sharp objects

• Scalpels, razor blades, broken glass
• Small cuts are 1 injury in lab classes
• Dispose all used sharp items into a sharps
  container
• Razor blades
• Any one time use glassware
• Broken glassware will be taken care of by helpers

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Safety rule 6 Avoid burns on hot items 7- Use
caution around electricity

• Burns caused by retrieving sample from boiling
  water is the 2 injury in classes.
• Watch for water near electric appliances.

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Safety rule 8- Clean up after yourself

• Reduce clutter
• Improve visibility
• Work efficiently
• Keep yourself and others safe

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Proper clean-up of biological materials

• Biological samples will degrade
• Degrading organisms attract bacteria, mold
• will ruin future experiments
• a biohazard
• smell

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Safety rule 9- No food or drinks in the culture
room!

• NO drinks, gum, mints, make-up.
• Saliva and crumbs from you can get on your work
  samples
• Contaminate your work
• Your work samples or their by-products can get on
  your food!
• And then into your mouth!!

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Invisible life forms are everywhere!

• Present on skin, fall off of hair, clothing, in
  saliva, tears, breath.
• Present on surfaces and in water
• Especially warm water

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Safety rule 10- Protecting your cultures
protects you!

• Biosafety hood (Class II safety cabinet)

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Aseptic Technique

• Prevents introduction of organisms into your
  cultures
• Roll sleeves up, pull hair back, remove rings,
  watches, bracelets
• Wash your hands
• 70 ethanol
• A spray-on hand sanitizer
• Gloves by suggestion
• Wash hands afterwards, gloves or not

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Safety rule 11- Proper biological disposal is
absolutely essential!

• For cell cultures (Biosafety level 1)
• Hazardous to dispose of any LIVING organism by
  dumping in trash or rinsing down the sink
• Bleach will kill any unused cells
• Squirt small amount into media and cells
• Wait a few minutes
• Rinse down sink with plenty of running water

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Proper disposal of biological waste

• Bacterial and fungal cultures
• Autoclave
• Use autoclave bags

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Other hazards in a bioengineering laboratory

• Compressed gases.
• Wear goggles when using compressed gas
• Cryogenic liquids
• To preserve cells
• Can cause burns

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