Center for Integrated Animal Genomics

1
Research Experience in Molecular Biotechnology
Genomics Summer 2008
Center for Integrated Animal Genomics
Whitney Davisab, Oliver Coutureb, Jolita Utheb,
Chris Tuggleb A Department of Biology, Alcorn
State University, Alcorn State, MS, 39096,
USA bDepartment of Animal Science, Iowa State
University, Ames, IA, 50010, USA
Analysis of the Porcine TAP1 promoter insertion
into pCR2.1 plasmid and looking for sequence
variants.
Materials and Methods
SNP in TAP1 promoter Sequence 115
GAGTTTGGG Sequence 134 GAGTCTGGG Sequence 142
GAGTC/TGGG
Figure 6 SNP in sequenced DNA

• Wang Y., Qu, L., Uthe, J.J., Bearson, S.M.D.,
  Khuar, D., Lunney, J.K., Couture, O.P.,
  Nettleton, D., Dekkers, J.C.M., and Tuggle, C.K.
  2007. Global transcriptional response of porcine
  mesenteric lymph nodes to Salmonella enterica
  serovar Typhimurium, Genomics. doi10.1016/j.ygeno
  .2007.03.018
• N. Hadjeb and G.A. Berkowitz, Preparation of
  T-over-hang vectors with high PCR product cloning
  efficiency, BioTechniques 20 (1996), pp. 2022
• Chen Q., Zhou H., Chen J., Wang X. 2006. Using a
  modified TA Cloning method to create entry
  clones. Analytical Biochemistry. Volume 358,
  Issue 1. 120-125
• Wei L., Sandbulte M., Thomas P., Webby R.,
  Homayouni R., Pfeffer L., 2005, NFkB negatively
  regulates interferon-induced gene expression and
  anti-influenza activity. J. Biol. Chem.,
  10.1074/jbc.M51328620

References
Program supported by the National Science
Foundation Research Experience for
Undergraduates DBI-0552371