Denville Scientific, Inc. www.densci.com

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Denville Scientific, Inc.www.densci.com

• EvoCycler EPx

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EvoCycler EPx

• Again combining
• experimental design
• rapid amplification
• end-point product analysis plus Real Time
• easy data recall and transfer from user to user
  worldwide
• all in one straightforward system
• from simple preparative amplification and
  product validations through to SNP analysis and
  genotyping
• the evocycler provides results,even faster.

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EvoCycler EPx

• achieves fast ramp and cooling rates
• 30 cycles in under 30 minutes
• uses air to heat and cool
• multiple Chemistries can be used
• Intercalating dyes or specific probing systems
  e.g. Hybeacons
• contains individual excitation and detection for
  each tube
• LEDs for excitation and photodiodies for
  detection
• small footprint means that little space is lost
  on the bench.
• (30cm x 16cm x 16cm)

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EvoCycler EPx

• Software

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Easy to use Software
EPx makes use of simple drag and drop features
that simplify the cycler programming
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Easy to use Software
EPx allows easy programming including Reverse
Transcriptase, Hot-starts and touch down/up
protocols
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Integrated Primer design
EPx software can select optimum primers from an
imported sequence
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Integrated Primer design
EPx software can allow automated oligo ordering
either via web or saved as a file for email or
fax ordering
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Integrated Primer design
EPx software will generate the optimum thermal
profile based on the imported sequence and
identified primer sets
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Sample input
EPx software allows input for sample name, plus a
text box for further sample information. Ability
to note standards and concentration is also
included
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Melt Curves
EPx generates melt curve data at the end of each
amplification identify your product, easily
discriminate amplification artifacts and primer
dimer evocyclers powerful auto-scoring
functions allow data to be automatically scored
to provide quick and simple product identification
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GelPhoto
the EvoCycler EPx uses proprietary software to
create a virtual gel image from the melt curve
data based upon melt peaks although gel
electrophoresis is the most widely adapted
technique for separating nucleic acids it has a
number of disadvantages compared with GelPhoto
which provides a repeatable, accurate method for
visualising amplification products in a format
that is easily understood
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Real Time
Real Time without the hassle
EPx creates Ct plots and allows the opportunity
to do Standard curves for Quantitation analysis
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Real Time visualisation
Fluorescence can be monitored in real time as the
reaction progresses, no need to wait until the
end to see you have forgotten to add a vital
reagent!
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Multiple Instruments

• Software senses when more units have been added

Multiple programmes can be run at the same time,
increased throughput or increased
flexibility Run different protocols at the same
time or start multiple runs at different times
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Software Summary
User friendly software Training is simple and
user can be confident with the software almost
instantly Stand alone analysis software is
available windows based software Contains Prime
r Design Experiment / instrument run Analysis
includes melt curves virtual gel Real Time
Quantitation Reports generated XML format for GLP
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EvoCycler EPx

• Hardware

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Qualities of EvoCycler EPx

• fast heating and cooling ramps
• Heating is 10C/sec (min 0.05C/sec)
• cooling is 5C/sec (min 0.05C/sec)

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EPx Advantages
The new EvoCycler EPx was designed around the
airflow. Void spaces were eliminated, turbulence
was decreased Importantly airflow was reversed
compared to the original unit Reversal of the
airflow has provided rapid cooling (5ºC
sec-1) Unit cools to within 5ºC of ambient within
20 seconds Very good heating rates
(10ºCsec-1) Air exiting the cycler is much
cooler
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Airflow
in
out
airflow widens out from the fan before it is
forced through a restricted passage past the
tubes, accelerating the airflow
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Heaters
Heaters redesigned for optimal performance at
110v Now a single unit with in-built balancing
heaters, so no cold spots and also no airflow
restrictions. Heater unit can be replaced easily
if required
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Excitation Detection systems
Evocycler uses LEDs for excitation and
Photodiodies for detection LED's offer good
mechanical stability, very low maintenance, a
long life (over 100,000 hours) and very low power
requirement. Photodiodes offer excellent
sensitivity, good mechanical stability, very low
maintenance, a long life (over 100,000 hours) and
very low power requirement. There are not the
problems associated with Tungsten lamps nor CCD
cameras
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Tube Optics
rubber o-ring alleviates requirement for heated
lid
parallel optics LEDs Photodiodies
Evotube held for correct optical alignment
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Qualities of EvoCycler EPx

• Easy to set up
• Can be installed within minutes, a simple USB
  connection from the instrument to the computer is
  all that is necessary
• Runs on Standard Mains power no specialised
  installation is necessary

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Qualities of EvoCycler EPx

• Easy to set up
• Automatic sensing of Voltage 110v 240v
• Additional units can be added using additional
  USB slots on computer or USB hub

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Qualities of EvoCycler EPx

• Software senses when more units have been added

Multiple programmes can be run at the same time,
increased throughput or increased flexibilty Run
different protocols at the same time or start
multiple runs at different times
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Transportable

• The small size of the Evocycler combined with
  its low power requirement means that it is
  perfect for use in the field.
• The Evocycler will run off a 12v car battery in
  conjunction with a 500 watt power inverter and is
  small enough to fit a camera case and can
  therefore be carried around unobtrusively.
• The ease of use of the software means that it
  can be operated by unskilled personnel whilst the
  autoscore function ensures that a 'yes-no' answer
  can be generated.