The e carriers:

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The e- carriers FAD FMN (1 or 2 e-) Q (1 or
2 e-) Fe-S centers (1 e-) Fe in cytochromes (1
e-) Cu (1 e-)
O2 ? H2O requires 4 e-
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What is the order of carriers in the chain?
increasing e- affinity
DGo -nFDEo -219 kJ/mol
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Oxidized carriers
Reduced carriers
order is as predicted by Eo values
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e- transport animation
vcell.ndsu.edu/animations/home.htm
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Entry of e- into the chain
some cyto. NADH
mito. NADH some cyto. NADH equivalents
from succinate
first step of b-oxidation
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NADH ? QH2
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(membrane-embedded portion)
Fig. 1. Architecture of the hydrophilic domain of
T. thermophilus complex I
L. A. Sazanov et al., Science 311, 1430 -1436
(2006)
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Complex II succinate dehydrogenase
Succinate ? QH2
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½ of Complex III
QH2 ? cyt C
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QH2 ? cyt C
complex III dimer has 4 binding sites for Q
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Q cycle (?) and H movement at complex III
animation 1st ½ of Q cycle
www.life.uiuc.edu/crofts/bc-complex_site/qo-site_m
ovie.html
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Core of complex IV
cyt C ? O2
e- carriers 2 hemes 3 Cu
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Note 2 H pumped/pair of e-
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