Vertebroplasty Material Analysis by Fourier Transform Infrared Spectroscopy

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Vertebroplasty Material Analysis by Fourier
Transform Infrared Spectroscopy

• Naomi Barth
• July 15, 2004
• Biomaterials Group
• Advisor Dr. Marcolongo

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What is Human Bone?

• 45 - 60 mineral Calcium Phosphate
• 20 - 30 matrix collagen
• 10 - 20 water

Schematic of the bone tissue.
Flahiff CM, Blackwell AS, Hollis JM, Feldman DS.
Analysis of a biodegradable composite for bone
healing. J Biomed Mater Res 199632419-424.
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Comparison of Osteoporosis Bone and Healthy Bone
Healthy Bone
Osteoporosis Bone
http//www.tarjetaweb.org/clientes/lopezolivares/i
mages/huesos.jpg
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Effect of Osteoporosis
http//www.inpensacola.com/fe/cityguide/seniors/ar
ticles/images/osteoporosis.jpg
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Methods Already in Use
Bone cement is injected into the fractured
vertebrae
www.nlm.nih.gov
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How do we replace bone ?
Calcium Phosphate
Collagen
BONE

Calcium Phosphate / Polymer Composite
Synthetic Calcium Phosphate
Polymer

• Tailor-made properties simulate natural bone
  characteristics
• Bioactivity at bonding site

Aoki H. Science and Medical Application of
Hydroxyapatite. JAAS 1991. 11-27, 99-107.
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Our proposed material
Material at room temperature
Material at body temperature
Photo reproduction was made under the courtesy of
Dr. Marcolongos Laboratory
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Engineering Design
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FTIR Fourier Transform Infrared
http//www.paris.ensam.fr/recherche/WEB-LTVP/image
s/Materiel/FTIR2.JPG
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Key Peaks for Identifying our Polymer Sample
1700
1000
FTIR result was made under the courtesy of Dr.
Marcolongos Laboratory
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Why FTIR is Important

• Identification of chemical structure (qualitative
  analysis)
• Calculation of chemical concentrations
  (quantitatively analysis)
• Verification of a successful polymerization
• Comparison of material to natural bone

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Further Analysis

• Comparison of the FTIR results of the following
  samples
• Injectable hydrogel polymer
• In vitro immersed hydrogel

My Final Goal is to master the FTIR technique, so
that I can apply it to my future research work.
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Acknowledgements

• Dr. Marcolongo
• Emily Ho
• Jon Thomas