Saccharomyces cerevisiae bakers yeast

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Saccharomyces cerevisiae(bakers yeast)
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Why Use Yeast

• Model Eukaryote
• Genetic Tractability
• Offers Advantages Unique to Microbial Organisms
  (growth, storage, genome manipulation etc)
• Biochemical and Metabolic Pathways Similar to
  Human Cells
• Genetically Defined (haploid/diploid etc)
  Laboratory Strains Available

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Saccharomyces cerevisiae

• 16 Chromosomes
• Genome completely sequenced 1996
• First single cell eukaryote genome sequenced
• Goffeau et al., Life with 6000 Genes, Science
  1996 274 546-567
• 12,068 kilobp (13 Mb)
• 5,885 potential open reading frames (ORFs)
  identified
• 140 genes encoding rRNA
• 40 genes encoding snRNA
• 275 genes encoding tRNA

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Gene Disruption in S. cerevisiae

• Why do gene disruption in yeast
• To help identify cellular function of gene
  product
• Biochemical/metabolic phenotypes
• Growth phenotypes
• Morphological phenotypes
• To determine if the gene is essential for
  viability

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WT vs. ?suc2 Yeast Strains
SUC2
Kanr
Normal (WT) strain No growth on media
containing G418 (Geneticin) Secretes invertase
(hydrolyzes sucrose)
?suc2 (SUC2 gene deleted and replaced with
kanamycin resistance gene) Normal growth on
G418 Invertase production abolished (does not
hydrolyze sucrose)
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Analysis of Yeast Strains using the polymerase
chain reaction (PCR)
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2,145
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2,055