Hemoglobin Experiment

1
Hemoglobin Experiment
In the hemoglobin experiment, blood was mixed
with potassium ferricyanide in a test tube. This
tube is then placed in a spectrophotometer (seen
above) and the amount of cyanmethemoglobin is
determined by measuring the amount of
transmittance (T) of light that passes through
the test tube. The T can be used to determine
the number of grams of hemoglobin that is in 100
ml of that blood. (See problem on slide 3.)
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3

• PROBLEM
• (answers are on following slide)
• 1. If the T is 44, how many grams of hemoglobin
  can 100 ml of this blood carry? (See chart on
  slide 2.)
• How many ml of O2 can this same blood carry?
• If the T is 30, how many grams of hemoglobin can
  100 ml of this blood carry? (See chart on slide
  2, look at the calculation at the bottom.)
• How many ml of O2 can this same blood carry?

4

• PROBLEM
• If the T is 44, how many grams of hemoglobin can
  100 ml of this blood carry? (See chart on slide
  2.)
• 14.3 g
• How many ml of O2 can this same blood carry?
• 14.3 g (Hgb) X 1.34 19.16 ml O2
• If the T is 30, how many grams of hemoglobin can
  100 ml of this blood carry? (See chart on slide
  2, look at the calculation at the bottom
• (100-30) x .256
  17.92 g
• How many ml of O2 can this same blood carry?
• 17.92 x 1.34 24.01 ml O2