Module 1B

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Module 1B
UNMC General Biosafety Training BSL-1 and BSL-2
Laboratories
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General BiosafetyModule 1 Outline

• Overview
• Module 1A
• Biosafety Level 1 and 2 Labs
• Module 1B
• Safety Equipment
• Module 1C
• Decontamination and Biohazardous Waste Handling
• Module 1D

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General BiosafetyModule 1B Synopsis

• Information about this training module
• For a comment or to ask a question, you will find
  a link on the main page of the course.
• To enhance the size of this PowerPoint
  presentation, click on the screen icon at the
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• Once this section is completed, click on the X
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  return to the main page to access Module 1C.

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Who needs this training?

• Individuals who work with or have access to
  biohazardous materials.

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Biosafety Levels of Containment

• Containment involves a combination of laboratory
  issues to include
• Standard microbiological practices
• Personal protective equipment
• Safety equipment
• Primary barrier
• Laboratory facilities
• Secondary barrier

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Biosafety Level 1 (BSL-1)
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BSL-1 - Overview

• Suitable for work involving well- characterized
  agents not known to cause disease in healthy
  adult humans and of minimal potential hazard to
  laboratory personnel and the environment.

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Examples of Organisms Requiring BSL-1

• Bacillus subtilis
• Saccharomyces cerevisiae
• Staphylococcus epidermidis
• Escherichia coli K-12
• (NIH Guidelines, Appendix B)
• (all are risk group 1 organisms)

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BSL-1 Standard Microbiological Practices

• Restrict or limit access when conducting research
• Prohibit eating, drinking and smoking
• Prohibit mouth pipetting

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BSL-1 Standard Microbiological Practices

• Needles sharps precautions
• Use sharps containers
• DONT break, bend, re-sheath or reuse syringes
  or needles
• Use alternatives to needles when available

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BSL-1 Standard Microbiological Practices

• Needles sharps precautions (cont.)
• DONT place needles or sharps in office waste
  containers

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BSL-1 Standard Microbiological Practices

• Use mechanical
• pipetting
• devices

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BSL-1 Standard Microbiological Practices

• Wash hands

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BSL-1 Standard Microbiological Practices

• Minimize splashes and aerosols
• Decontaminate work surfaces daily
• Decontaminate infectious waste
• Maintain an insect rodent control program

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BSL-1 Safety Equipment (Primary Barrier)

• Personal protective equipment
• Face shield
• Eye wear
• Gloves
• Lab coat

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BSL-1 Facility Design (Secondary Barrier)

• Countertops and floors easily cleaned and
  decontaminated

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BSL-1 Facility Design(Secondary Barrier)

• Requirements
• Lab Location -not separated from other lab
  sections
• Structure -normal construction
• Ventilation -no specific requirements

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BSL-1 Training Requirements

• Supervisor
• Trained in microbiological or related science
  practices
• Lab personnel
• Specific training in lab procedures

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Biosafety Level 2 (BSL-2)
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BSL-2 - Overview

• Suitable for work involving agents of moderate
  potential hazard to personnel and the environment.

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Examples of Organisms Requiring BSL-2

• Measles virus
• Salmonella spp.
• Toxoplasma spp.
• Hepatitis B virus
• (NIH Guidelines, Appendix B-II)
• (all are risk group 2 organisms.)

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BSL-2 Standard Microbiological Practices

• Follow all practices used for BSL-1 containment

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BSL-2 Safety Equipment (Primary Barrier)

• Class II Biological Safety Cabinet
• To protect product, personnel, and the
  environment.
• But.only recommended
• Not required for BSL-2 work

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BSL-2 Safety Equipment (Primary Barrier)

• Class II Biological Safety Cabinet
• Equipment is laid out to not restrict airflow in
  the cabinet

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BSL-2 Facility Design (Secondary Barrier)

• Requirements
• Laboratory has lockable doors
• Sink available for hand washing
• Work surfaces easily cleaned
• Bench tops are impervious to water
• Sturdy furniture

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BSL-2 Facility Design (Secondary Barrier)

• Requirements
• Adequate illumination
• Air flows into lab without re-circulation to
  non-lab areas
• Windows fitted with fly screens

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BSL-2 Facility Design (Secondary Barrier)

• Signage on the door with restricted access
  when work in progress.

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BSL-2 Facility Design (Secondary Barrier)

• BSL-1 Facilities PLUS
• Decon method
• Autoclave may be available
• Off-site program
• Eyewash station present

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BSL-2 Facility Design(Secondary Barrier)

• Requirements
• Location - separated from public areas
• Structure - normal construction
• Ventilation - directional

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BSL-2 Training Requirements

• Supervisor
• Is a competent scientist
• Restricts access
• Lab Personnel
• Aware of potential hazards
• Proficient in practices/techniques

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BSL-3 and BSL-4 Containment

• BSL-3 containment is discussed in Module 2.
• BSL-4 containment is not available on the
  UNMC/UNO campuses.

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Biological Spill Clean-Up

• All laboratories should have posted an emergency
  plan
• To handle accidental spills
• To handle personnel contamination
• UNMC policy IBC04 is available on the IBC
  website for information
• www.unmc.edu/ibc/

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Reporting of Research Related Adverse Events

• Research related adverse events include
• Biological spills
• Exposure to biohazardous agents
• Non-adherence to NIH Guidelines
• All such events must be reported to the IBC
• Refer to UNMC policy IBC24 for information and a
  reporting form
• www.unmc.edu/ibc/
• Biosafety Officer can be contacted for additional
  information

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This completes Module 1B

• Click on the X at the top of the page to close
  this window. Then
• Continue on with Module 1C or
• Comment or ask questions about Module 1B by
  clicking on the link on the main page of the
  course