Bacterial Transformation Teacher Preparation Sterile Materials

1
Bacterial TransformationTeacher
Preparation Materials Lists
2
Materials List-Item 1(per group of students)

• 1 aluminum foil packet containing 4 sterile paper
  clips that are large and smooth. The clips should
  be opened into a 90 angle and the small end bent
  to close it.

Bacterial Transformation Teacher Preparation
3
Materials List -Item 2(per group of students)

• 1 aluminum foil packet containing 4 sterile paper
  clips that are small and smooth. Bend one end of
  each paper clip into a loop.

Bacterial Transformation Teacher Preparation
4
Materials List -Item 3(per group of students)

• 2 microcentrifuge tubes (1.5 ml) containing 2
  drops of sterile CaCl2 and labeled "CaCl2"
• Place on ice in a refrigerator until ready to
  use.

Bacterial Transformation Teacher Preparation
5
Materials List -Item 4(per group of students)

• 1 petri dish containing only nutrient agar and 1
  dish with ampicillin labeled "No Amp" on the
  bottom

Bacterial Transformation Teacher Preparation
6
Materials List -Item 5(per group of students)

• 1 petri dish containing nutrient agar and 1 dish
  with antibiotic ampicillin labeled "Amp" on the
  bottom

Bacterial Transformation Teacher Preparation
7
Materials List -Item 6(per group of students)

• Copies of the laboratory instructions, one for
  each student

Bacterial Transformation Teacher Preparation
8
Materials List -Item 7(per group of students)

• 1 glass bottle with a cap containing sterile
  nutrient broth and labeled "LB Broth"

Bacterial Transformation Teacher Preparation
9
Materials List -Items 89 (per group of students)

• 1 Sharpie marking pen
• 1 rubber bulb for the pipettes

Bacterial Transformation Teacher Preparation
10
Materials List -Item 10(per group of students)

• 1 aluminum foil packet containing the glass parts
  of 4 sterile pasteur pipettes or sterile plastic
  pipettes

Bacterial Transformation Teacher Preparation
11
Materials List -Item 11(per group of students)

• 1 aluminum foil packet containing 8 sterile
  toothpicks

Bacterial Transformation Teacher Preparation