Microbiology 156 http:chsweb'lr'k12'nj'uspsidelsky

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Microbiology 156http//chsweb.lr.k12.nj.us/psidel
sky

• Mrs. Sidelsky
• psidelsky_at_lrhsd.org
• psidelsky_at_bcc.edu

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Class One

• Please sign safety forms
• Make sure you understand the safety rules
• Be able to identify safety equipment in the room

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Dress for Laboratory
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Laboratory

• Laboratory coat is required
• Hair should be tied back
• No open shoes( sandals)
• No Gum, food, beverages
• Keep personal belongings on shelves at the
  entrance to the laboratory
• Prepare for each class by reading laboratory
  experiment(s) for class

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Journal

• For the second class you will need a Journal
• The Journal can be a marble notebook or spiral
  bound notebook. It will consist of laboratory
  experiments for each class.
• Directions for your laboratory manual will be
  provided during the second class

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Compound Light Microscope
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Introducing your microscope

• Always clean lenses before starting
• Always with both hands supporting base
• Store with arm facing the door. Cover
• Cleaning lenses before and after using
• Focus with scanning and low before proceeding to
  high and oil
• Learn the most effective ways to regulate and
  manage light

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Objective Lenses
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Microscope terms

• Microscope terms
• Magnification
• Total magnification
• Resolution
• Depth of field
• Size of field
• Working distance
• Parfocal
• Parcentric

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Microscope and Slides

• Three Threads
• Place slide on mechanical stage.  Make sure that
  stage clips are holding slide securely. 
• Use the scanning lens( red) to find the point at
  which the threads cross.
• Focus the microscope .  Using the coarse
  adjustment
• Observe and draw
• Change to low power. Turn the revolving nose
  piece so that the yellow lens is in place.
  Observe and draw

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Observations

• What do you notice about the size of field?????
• What do you notice about your ability to focus.
• Focus up and down very carefully and slowly with
  the fine adjustment
• What occurs? Why

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High Power

• If your focus is sharp and clear, turn the
  revolving nosepiece to blue
• Use the fine adjustment, focus carefully you
  are very close to the slide.
• Observe and draw

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Bacterial Slide

• Take a slide of a bacillus from the supply area
• Place it on the Stage
• Repeat the procedure, for focusing
• Adjust the light and draw
• Observe and draw.

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Bacterial shapes
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Bacterial Shapes and Arrangement
Streptococci
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Rods- Bacilli Stained with Gram Stain