Small Animal Imaging Core

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Small Animal Imaging Core
Jerry D. Glickson, Ph.D.
University of Pennsylvania Department of
Radiology
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Magnetic Resonance Imaging Spectroscopy

• Instrumentation
• Varian Inova 4.7T/50 cm bore MRI/MRS system with
  Magnex gradients
• mice, rats, rabbits, dogs and small primates
• Varian Inova 9.4 T/8.9 cm bore MRI/MRS system
  with GE and Magnex gradients
• solutions, tissue extracts, perfused cells
  organs, mice, solids

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Magnetic Resonance Imaging Spectroscopy

• Instrumentation
• Varian Inova 9.4 T/31 cm bore MRI/MRS system
• mice, rats, rabbits, dogs and small primates

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Magnetic Resonance Imaging Spectroscopy (Cont.)

• Clinical Research Instrumentation
• 1 Siemens Sonata 1.5 T clinical scanners
• 1 Siemens Trio 3T clinical scanner
• 1 Home Built 2T whole body scanner for in-magnet
  exercise experiments this instrument is being
  replaced with a 3T commercial instrument that
  will be used exclusively for research.

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Optical Imaging

• Visible light is primarily absorbed or reflected
• Light guides can be introduced into body cavities
• Near infrared has a window 780-900 nm2.5-5 cm
  penetration depth (Britton Chance)
• Oxy- and deoxyhemoglobin
• Respiration
• Neuronal stimulation
• Cognative stimulation
• Near IR dyes
• Conjugated for delivery to specific organs and
  tissues
• Targeted at specific molecules
• mRNA
• Specific proteinases, etc.
• Key advantages cheap, portable, versatile

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Optical Imaging

• ART Imaging System(Wafik El-Deiry)
• Detects endogenous exogenour NIR fluorophores
• Detects luciferase marker gene
• Detects GFP, YFP, RFP
• Tomographic
• Luminescence Imaging (Wafik El-Deiry)
• Detects luciferase marker gene
• Detects GFP, YFP, RFP
• Currently non-tomographic

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PET SPECT (Joel Karp)

• High contrast and sensitivity
• mPET (mice, rats and cats) 2 mm resolution
• clinical PET 4 mm resolution
• mSPECT 1 mm resolution
• Can be targeted to specific tissues and
  pathologies
• Labeled antibodies
• Gene expression
• Receptors -- Her2/Neu, somatostatin, etc.
• Apoptosis
• High sensitivity (nanomolar)

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CT Imaging (Peter Joseph, Felix Wehrli)

• High anatomical contrast
• Bony structures
• Lungs
• Air passages?
• Can be coregistered with PET or SPECT
• Rapid
• Less contrast in soft tissues
• Radiation

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Ultrasound (Sandy Sehgal)

• Blood Flow Measurements
• Labeled antibodies in the blood stream
• Anatomical structures
• High Frequency Commercial Animal Imager
• Key Advantages
• Cheap
• Portable
• Noninvasive
• Disadvantages
• Image resolution is limit