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Switching nonlinear signals

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Fluorescence microscopy. Switching fluorescence off. Stimulated emission. Useful! ... STED microscopy. Prof. Stephan Hell, G ttingen. Nonlinearities. Coherent ... – PowerPoint PPT presentation

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Title: Switching nonlinear signals


1
Switching nonlinear signals
2
Light in matter
3
Spectroscopic transitions
4
Fluorescence
5
Fluorescence microscopy
6
Switching fluorescence off
Stimulated emission
7
Useful!
Prof. Stephan Hell, Göttingen
8
STED microscopy
Prof. Stephan Hell, Göttingen
9
Nonlinearities
10
Coherent nonlinear signals
11
Coherent nonlinear signals
12
CARS microscopy
13
CARS microscopy
100 ?m
14
Switching?
CARS
15
Messy business!
CSRS
16
Even messier!
a total of 28 different interactions between the
colors are present!
17
How does the signal grow?
18
Coupled equations
19
Phase matching
20
Depletion
CARS intensity
z
21
Phase-matching in microscopy
1 ?m
22
Controlled depletion?
23
Switching with an external field
24
Vibrational oscillators
25
Switching in practice
Rel. CARS intensity
26
Fun with switching
27
What have we learned?
A very weak external field can be used to switch
off coherent nonlinear signals
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