Simple HighPerformance Biochemical Analysis

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Simple High-Performance Biochemical Analysis

• David Ross, Physicist
• NIST
• Gaithersburg, MD

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Technology Summary

• Method for chemical/biochemical analysis
• Quantitative measurement of components of a
  sample (blood, urine, water)
• Our technologies combine multiple analytical
  steps into one simple method.

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Conventional Microfluidic Analysis
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Conventional Microfluidic Analysis
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Conventional Microfluidic Analysis
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Our Approach Simplicity
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Our Approach Simplicity

• Simple, short device combines
• Separation, Concentration, Sample Prep
• Simplicity Reliability

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Our Approach Simplicity
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Technology Description

• Simple, short microchannels
• No complicated microfluidic structures
• No injection
• No voltage switching
• Combines multiple analytical steps into one
• Concentration
• Separation
• Sample preparation.
• Electric field plus controlled counter-flow
• Proof of concept done for wide range of analytes
  and applications

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Applications

• Analysis Tool
• Proteins, amino acids, DNA, nanoparticles,
  environmental contaminants, drugs, salts
• Estimated market for microfluidic diagnostics in
  5 years 6.5 Billion
• Potentially disruptive technology, enabling
  microfluidics for
• Point of care diagnostics (16 Billion by 2010)
• Environmental/Process monitoring (6 Billion in
  1995)
• Pharmaceutical analysis

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Competition

• Conventional Capillary Electrophoresis
• Beckman Coulter
• Agilent
• Groton Biosystems
• Prince Technologies
• Lumex
• Microchip Electrophoresis
• Agilent
• Caliper Life Sciences
• Micronit
• Nothing else that combines concentration and
  higher performance separation in a simple device

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Current Status

• Patents
• One issued patent (U.S. 7,029,561)
• Four non-provisional applications (20050258040,
  20050145495, 20040206626, one submitted last
  week)
• Ready for Licensing
• NIST to continue applications development
• Trace measurements/standards applications
• Clinical application
• Planetary exploration

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Next Steps

• Extensive proof of concept work done
• Development of prototype instrument
• Implementation with different detectors
• Buffer solution development

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Contact

• For more information
• David Ross
• National Institute of Standards and Technology
• 301-975-2525
• david.ross_at_nist.gov
• Website URL