LSRII Flow Cytometer

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LSRII Flow Cytometer
Central Flow Cytometry Facility

• Multi Laser
• 4 Lasers, Blue, Red, Violet and UV
• Multi Parameter
• FSC, SSC,
• 6 Channels from Blue
• 2 Channels from Red
• 2 Channels from Violet
• 2 Channels from UV
• Digital Acquisition
• 255000 levels as opposed to 1024
• Compensation on or off line

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Overview
Octagon
Trigons
Blue Laser
Light Guide
Red Laser
Violet Laser
UV Laser
FSC Detector
Flow Cell
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Octagon Optics
SSC
Filter
Dichroic
Emitted light From 488nm Laser
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Trigon Optics
Only 2 channels are populated
Emitted light From 350nm Laser
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Detail of Optics
Long Pass Dichroic Mirror
Bandpass Filter
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Flow Cell
Sheath Fluid surrounds and Focuses Cells
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Flow Cell