The Difference Between Absolute Quantification and Relative Quantification

1
The Difference Between Absolute Quantification
and Relative Quantification

• Summary
• As the development of technology, there are more
  and more methods in the biological field.
  However, these methods, such as absolute
  quantification and relative quantification, can
  confuse scientists and students. Here I will
  introduce the difference between the two methods.

2

• Body
• Absolute quantification
• The purpose of absolute quantification is to
  detect the number of molecular in the sample of
  aimed genes, which is called copy number. However
  the aim of the relative quantification is to
  detect the relative proportion of aimed genes in
  two or more samples. And there is no need to know
  the copy number in every sample.
• Differences in experiement
• In the experiemnet of the absolute
  quantification, it has to need the standard
  sample with the known copy number and the
  standard curve, which is not a big deal in the
  relative quantification experiement. There are
  two methods in relative quantification---standard
  curve and the aCT value comparation. If we use
  the first method, firstly, we should find out the
  absolute standard samples or the relative
  standard samples, which is much more convenient
  and it is easily to be operated. Whats more,
  relative quantification samples just need the
  proportion of DNA and RNA in samples without
  knowing the molecular number of standard samples.
  There is a typical method is to make the the
  known pg number sample become a series of
  gradient dilution. The CT value comparation
  method is a little complicated.
• The data of the absolute quantification is easily
  to be understood. However, the preparation of
  samples and the detection of DNA consistence are
  a little difficult. You can solve this problem by
  buying the commercial reagents box from
  marketing. The standard sample of relative
  quantification can be made in labs by yourself.
  However, there are a lot of difficulties in
  handling the data and explaining the data.

3

• Here I will conclude the whole points for you.
  The relative quantification is really convenient
  and cheap. However, it does not have the specific
  model and the high sensitivity. It is always
  being applied to the differentially expressed
  genes, which is the most commonly used way for
  scientists. And the advantage of absolute
  quantification is laying in the high degree of
  soecificity and great repeatability. However, it
  is just suitable one determined sample and it is
  not cheap. And the absolute quantificationis
  always applied to determin the genes copy number,
  which is used in clinical diagnostic, such as,
  virus detection and nosophyte detection.
• About the athor
• Relative quantification and the absolute
  quantification are really important in the
  biological and chemical fields. Creative
  Proteomics has contribute a lot in this field,
  especially the absolute quantification.