Metagenomics sequencing - PowerPoint PPT Presentation

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Metagenomics sequencing

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Metagenomics is the study of metagenomes, genetic material recovered directly from environmental samples. The broad field was referred to as environmental genomics, ecogenomics or community genomics. Recent studies use "shotgun" Sanger sequencing or next generation sequencing (NGS) to get largely unbiased samples of all genes from all the members of the sampled communities. – PowerPoint PPT presentation

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Title: Metagenomics sequencing


1
The metagenomics sequencing
CD Genomics
2
Metagenomics
Metagenomics is the study of metagenomes, genetic
material recovered directly from environmental
samples. The broad field was referred to as
environmental genomics, ecogenomics or community
genomics.
CD Genomics
3
Basic strategies
sample of environment
enrichment culture
extraction of metagenome
Ectopic extraction
Plasmid extraction
carrier Fosimid, Cosmid, BAC, ? phage
Host escherichia coli, streptomycete,
pseudomonas and etc.
metagenomic library construction
metagenomic library screening
sequence-based screening
nonsequence-based screening
New gene activity expression
CD Genomics
4
Database for Metagenomics studies
UniMES The UniProt Metagenomic and Environmental
Sequences (UniMES) database was developed as a
repository for metagenomic and environmental
data. Ebi Metagenomics Submit, analyse,
visualize and compare your data. MEGAN MEGAN is
a versatile tool for analyzing taxonomic and
functional content of metagenomes. It allows
comparative analyses of different
datasets. Integrated Microbial Genomes (IMG)The
mission of the Integrated Microbial Genomes (IMG)
system is to support the annotation, analysis and
distribution of microbial genome and metagenome
datasets sequenced at DOE's Joint Genome
Institute (JGI). Ribosomal RNA database 5S
Ribosomal RNA DatabaseAn integral component of
the large subunit of all cytoplasmic and most
organellar ribosomes. GreengenesProvides
quality-controlled microbial DNA data and tools
to researchers for rapid analysis of
user-generated sequences. 5S RNAdb A database
dedicated to nucleotide sequences of bacterial,
archaeal and eukaryotic (cytoplasmic and
organellar) 5S ribosomal RNAs and their genes.
RDPProvides ribosome related data and services
to the scientific community, including online
data analysis and aligned and annotated Bacterial
and Archaeal small-subunit 16S rRNA sequences.
CD Genomics
5
CD Genomics offers a complete range of
metagenomics solutions
16S rDNA tagging general taxonomics
classification Whole genome metagenomic
sequencing Establish microbial gene catalogue
Human microbe related disease association
analysis Metatranscriptome survey
CD Genomics
6
Application
Analyze the relative abundance of microbial
species under varying environmental conditions
Discover new genes and make functional
predictions in unculturable species Perform
gene expression analysis and functional
annotation within microbial communities
Identify novel pathogens in viral outbreaks by
sequencing fragments of amplified RNA from
infected individuals Take advantage of the
long read lengths to generate the most accurate
representation of samples
CD Genomics
7
Our 16s sequencing is faced with yogurt,
lactobacillus, and other manufacturers in the
world. 18s sequencing is faced with brewers.
CD Genomics
8
About us
CD Genomics was established in 2004, we are
aiming at providing the research community with
high quality Next Generation Sequencing, high
throughput microarray services. Due to the demand
for our services has being increased CD Genomics
has already updated its technology platform to
mainstream NGS and microarray instruments. At
present, our senior bioinformaticians have ever
viewed more than ten thousands of trace files and
accumulated abundant experience with our Illumina
HiSeq2000/2500, Illumia Miseq, Ion Torrent PGM,
PacBio RS and ABI 3730/3730XL analyzers. We
continue to work hard to offer you the same
dependable services to pharmaceutical and biotech
companies, as well as academia and government
agencies for the purpose of satisfying all your
sequencing or array needs. In 2007, CD Genomics
has expanded its services to molecular biology
research and its development needs. We have
become a service provider in sequencing,
microarray analysis, library construction and
genotyping. Our progress could not be achieved
without our large group of client's
support. Through nearly ten year's hard working
and depend on our professional work team, we are
proud of satisfying the needs of our clients both
at home and abroad, which across more than 50
countries and districts. We always devote
ourselves to providing you with the best and
professional service.
CD Genomics
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