Renaturation of Recombinant Proteins

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Renaturation of Recombinant Proteins
Creative BioMart
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Recombinant protein expressed in bacteria often
form inclusion bodies, especially when they are
expressed at high levels. Creative BioMart
retrieves expressed GST fusion protein in soluble
form after lysis and extraction procedures.
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Advantages

• Specifically for solubilizing and renaturing GST
  inclusion bodies.
• Time saving without lengthy dialysis or dilution
  step.
• No pH variation and Redox Pair involved.

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Refolding of the solubilised proteins is
initiated by the removal of the denaturant. The
efficiency of refolding depends on the
competition between correct folding and
aggregation. To slow down the aggregation process
refolding is usually carried out at low protein
concentrations, in the range of 10-100 mg/ml.
Furthermore, refolding conditions must be
optimized for each individual protein.
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Methods for the refolding of proteins

• Dialysis.
• Slow dilution.
• Rapid dilution.
• Pulse renaturation.
• Chromatography reagent.

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Creative BioMart has been successfully refolded
many recombinant proteins. We have accumulated a
lot of experience in renaturation of recombinant
proteins as inclusion bodies. Creative BioMart
can provide professional technical service for
you.
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BioMart Address 45-1 Ramsey Road Shirley, NY
11967, USA Email contact_at_creative-biomart.com W
ebsite http//www.creativebiomart.net