Thin Layer Chromatography

1
Thin Layer Chromatography Applications and
Advantages
2
Thin Layer Chromatography

• Thin layer chromatography is a type of liquid
  chromatography that is used to separate
  non-volatile mixtures such as glycerin into its
  individual components. The process is performed
  on a sheet of glass, plastic, or aluminium foil
  coated with a thin layer of adsorbent material
  like silica gel, aluminium oxide (alumina),
  or cellulose (known as the stationary phase).
• However, TLC is utilized in many industries for
  different purposes.

3
Applications Advantages of Thin Layer
Chromatography (TLC)
4
Applications of (TLC) are

• Test the purity of the sample Thin layer
  chromatography helps to detect the purity of the
  sample by direct comparison with the standard or
  authentic sample. Any impurity in the sample
  shows up as extra spots in chromatography.
• Identify the components Thin layer
  chromatography can purify, isolate and identify
  the natural products like volatile oil
  or essential oil, fixed oil, waxes, terpenes,
  alkaloids, glycosides, steriods etc. in the test
  samples.

5

• Examination of reaction mixtures When you
  combine two or more substances and the resultant
  combination shows a change in the structure or
  energy content of the interacting entities, the
  final product is called a reaction mixture. When
  such a mixture is subjected to thin layer
  chromatography, it can tell whether the reaction
  is complete or not and also check other
  separation and purification processes
• Biochemical analysis Biochemical metabolites
  from the body fluids, blood plasma, serum, urine
  etc. can be isolated using thin layer
  chromatography.
• In chemistry, TLC is used to separate and
  identify closely related compounds or cations and
  anions in inorganic chemistry.

6

• Pharmaceutical industries utilize TLC technique
  for qualitative analysis or detect impurities in
  various medicines like hypnotics, sedatives,
  anticonvulsants, tranquillisers,
  anti-histaminics, analgesics, local anaesthetics,
  steroids, etc. Yet another important application
  of TLC is to separate multi-component pharmaceutic
  al formulations into its individual components.
• In food and cosmetic industry Any artificial
  colour, preservatives, sweetening agent, and
  other impurities in food and cosmetic products
  can be detected and isolated by TLC technique.

7
Advantages of (TLC) are

• It requires less equipment.
• The separation of components is faster, thus,
  saving time.
• The technique is highly sensitive and gives more
  accurate results.

8

• The lower detection limit of analytical sample in
  TLC is approximately one decimal lower than that
  of paper chromatography. Further, TLC requires
  very small quantities of a sample to analyse and
  give results. This proves cost-effective for labs
  facing scarcity of funds.
• In TLC, you can spray corrosive agents to
  identify the sample, but this is not possible in
  paper chromatography as cellulose gets destroyed.
• Adsorption, partition, and ion exchange chromatogr
  aphy also utilize this technique as wide range of
  adsorbents are available.

9

• The individual samples do not get diffused as in
  paper chromatography.
• TLC separates the sample into its individual
  components. These components can be recovered
  easily by scratching the powdery coating of the
  plate. Moreover, quantitative separation of spots
  is possible with TLC.
• You can see and identify the individual
  components by UV light as the inorganic adsorbent
  background does not fluoresce.

10
(No Transcript)
11
To Know More
Contact Us Email ID project_at_cognibrain.com Phon
e 044-49595223 Visit us https//www.cognibrain.
com/