Macrophage Polarization

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Macrophage Polarization

• https//www.creative-biolabs.com/car-t/

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Background
Macrophages belong to a heterogeneous population
of innate myeloid cells which is involved in
disease and health. They are the most functional
diverse cells of the hematopoietic system.
Macrophages exert multiple functions in human,
including responding to pathogens and modulating
the adaptive immune response, induction and
resolution of inflammation, tissue repair, and
homeostasis. Macrophages own appealing plasticity
characteristic. Therefore, in response to
different stimuli, different populations of
macrophages with various physiological and
pathological roles can be developed. Creative
Biolabs offers the macrophage polarization assay
to induce different macrophage subsets.
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Assay of Macrophage Polarization
Macrophages reveal specific phenotypes and
functions in response to different triggers. In
the macrophage polarization assay, we provide
specific stimuli co-cultured with isolated bone
marrow-derived or peritoneal macrophages to
generate different polarized macrophage subsets.
Depending on the types of stimuli that
macrophages are exposed to, they are able to
polarize to M1 (pro-inflammatory) or M2
(anti-inflammatory) macrophages (see Fig 1).
Based on the stimuli and the achieved
transcriptional changes, the M2 macrophages can
be classified into four subdivisions - M2a, M2b,
M2c, and M2d. In this assay, except for different
macrophage polarization by adding certain
stimuli, we also provide support for our clients
with verification of each polarized
macrophage-based on the distinctly expressed
surface markers and/or produced cytokines.
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Here we list the different stimuli used to
trigger different macrophage populations.
? M1 macrophage IFN? and LPS? M2a macrophage
IL-4 or IL-13? M2b macrophage Immune complexes
and LPS? M2c macrophage Glucocorticoids or
IL-10? M2d macrophage Adenosines or IL-6
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Figure 1 Macrophage polarization.
(Zanluqui, et.al. 2015)
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Highlight Features
? Senior Ph.D. scientists and experienced team?
Skillful technologies and advanced platforms?
High quality but competitive price? Timely
after-sales service ? Customized assay design
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About Creative Biolabs

• With the exponentially increased demand in modern
  novel medicine, the CAR and TCR immune cell
  therapy has been one of the most promising
  approaches for target-specific cancer treatment.
  With integrated global resources in all aspects,
  Creative Biolabs provides one-stop full CAR T/NK
  Cell therapy development service, including early
  non-clinical research, process development,
  pre-clinical animal model testing, cGMP
  manufacturing, and phase I-III clinical trials.

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Contact

• 45-1 Ramsey Road, Shirley, NY 11967, USA
• Email marketing_at_creative-biolabs.com
• www.creative-biolabs.com/car-t

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