Diabody

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Diabody
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Diabody
What is Diabody?
Diabody is a noncovalent dimer of single-chain Fv
(scFv) fragment comprised of two chains expressed
separately, each of which consists of a VH from
one antibody and a VL from another antibody.
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To improve the efficiency of the
heterodimerization process, disulfide bond or
knobs-and-holes is introduced into the variant
domains. In addition, by tandem linking genes in
each chain with internal linker, four VH and VL
domains can be expressed in tandem and folded as
single chain diabody (scDb), which is also an
effective strategy for bispecific antibody
producton. Furthermore, fusing recombinant
variable domains to an Fc region or CH3 domain
(scDb-Fc and scDb-CH3) can not only help the
chain-pairing process, but also double the
valency of the final product. Meanwhile, the
increased size can also significantly prolong the
half-life of Db in serum.
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THANKS
Resource https//link.springer.com/referencewor
kentry/10.10072F978-3-642-16483-5_1603 https//ww
w.creative-biolabs.com/bsab/category/diabody-29.ht
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