in vivo animal testing

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CAR-T Preclinical In Vivo Assay

• Reference https//www.creative-biolabs.com/car-t/
  car-t-preclinical-in-vivo-assay.htm

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Introduction

• CAR-T cells are autologous or allogeneic T cells
  targeting specifically to antigens or markers
  expressed by tumor cells. In vivo assessment is
  of pivotal importance for investigating the
  factors that may affect the efficacy of CAR-T
  cells. The approaches of delivering CAR genes
  into T cells, the in vitro culture conditions,
  the constituents of the CAR construct, and the
  types of host T cells all have great impacts on
  CAR-T efficacy and safety. Data from in vivo
  assays is valuable for CAR-T optimization.

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What can CAR-T cells do?

• CAR-T cells capable of recognizing various
  tumor-associated antigens (TAAs) have been tested
  in many clinical trials and received plenty of
  encouraging results. However, several potential
  risks such as cytokine storm have also been posed
  for CAR-T cell clinical application. Therefore,
  preclinical in vivo assessment of the efficacy
  and safety of CAR-T cells are essential for
  dosage determination and risk managements for
  this promising immunotherapy against cancers.

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How to conduct in vivo assays?

• CAR-T cell preclinical in vivo animal testing
  performed in xenograft murine models derived by
  well-defined cell lines or primary human tumor
  cells.
• Immunodeficient mice, lacking of effective innate
  immunity for the absence of B cells, T cells, or
  NK cells, are the optimal animal models for
  engraftment of tumor cells and evaluation of
  CAR-T cells.
• The progression and remission of tumor cells
  detected in any body part throughout the whole
  treatment duration, obtaining an unambiguous
  evaluation of the therapeutic efficacy of CAR-T
  cells.
• A series of studies to investigate several
  potential safety concerns of CAR-T therapy
  including inappropriate immune activation
  (cytokine level), the potential for
  tumorigenicity, the risks of the delivery
  procedure, etc.
• The in vivo expansion, survival, persistence,
  migration, and distribution of the CAR-T cells
  are also systematically studied.

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Featured in vivo Assay Categories

• Construction of Xenograft Animal Model
• Strategies to Harness Cytokine Release Syndrome
• Efficacy Test of CAR-T
• Viability and Bio-distribution Study of CAR-T
• Toxicity Evaluation of CAR-T
• Quality Control for in Vivo Assays

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Targets for CAR-T Preclinical in vivo Assays
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Thanks

• Reference https//www.creative-biolabs.com/car-t/
  car-t-preclinical-in-vivo-assay.htm