Recombinant DNA Technology - Applications

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Recombinant DNA Technology Applications
R. C. Gupta M.D. (Biochemistry) Jaipur,
India
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Mapping of genomes
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Genomic library
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cDNA library
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Techniques to locate a desired
DNA insert in the vector
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Human Genome Project (HGP)
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Why to map the human genome?
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The challenge
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The beginning
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Aims and objectives of HGP
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The project was split into three phases of
five years each
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The basic steps were
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The results
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Production of proteins
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Synthesis of recombinant human
insulin
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Diagnosis of genetic diseases
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Medico-legal applications
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Mini-satellites
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DNA from crime scene
DNA of suspect 1
DNA of suspect 3
DNA of suspect 2
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Gene therapy
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Transgenic animals
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Knockout animals
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Gene therapy in human beings
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Commercial gene therapy
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Thank you