Thermal Cycler Machines Supplier

1
Factors To Consider When Purchasing A Thermal
Cycler Machine
2
A laboratory that specializes in DNA
amplification and polymerase chain reactions is
incomplete without a thermal cycler. Also known
as thermo cyclers or PCR machines, thermal
cyclers are an indispensable tool in the field of
molecular biology.
3
The machine consists of a heating block that
contains depressions in which the test tubes are
placed. The samples undergo a step by step
process of initial denaturation, annealing,
extension, final extension, and holding.
4
The important function of the cycler is to change
the temperature of its interior container at
specific times for particular durations. These
temperature changes allow for the creation of
multiple copies of the DNA fragment.
5
These are available in various models and sizes,
ranging from mini, personal models to compact and
even large sized machines. For extremely large
systems, thermal cycler manufacturers link many
PCR machines together with a centralized computer.
6
As one can imagine, the applications for thermal
cyclers machines are numerous. They can be used
in life science research, pharmaceutical
engineering, animal diagnostics, medicine, and
food testing, amongst others.
7
Type of Vessel and Capacity The heating blocks
in thermal cyclers are designed to store a number
of DNA samples. The block can be designed to
house DNA samples in small and large capacities
up to 300 samples. The most common container
type is for the 0.2 ml PCR tubes.
8
However, there are other containers designed for
0.5ml or even large conical shaped tubes. The
metal used to fabricate the heating block also
makes a difference to the operation. Silver is
the most preferred metal as it conducts heat and
cold better than most metals.
9
Block Temperature The precision with which the
thermal cycler changes temperature in the heating
block is paramount to its successful use. The
temperature needs to change uniformly and quickly
to provide accurate results.
10
Check whether the PCR machine has a Peltier
element for temperature control. This will
confirm that machine will be able to
instantaneously change temperatures during the
DNA amplification process.
11
Heated Top Cover Many a time, the PCR Thermal
Cycler process results in the reaction fluids
evaporating and creating condensation on the top
of the sample lids. This can lead to insufficient
data and results, causing a great delay in the
work.
12
Versatility Research and testing has helped
manufacturers to provide a number of flexible
solutions, which has made the thermo cycler more
versatile in its use. Early versions of it had
one solid heating block. Today, the blocks are
designed to be interchangeable, enabling for
faster DNA amplification of vials of various
sizes.
13
Another option offered to customers is modular
heating blocks. This option allows for the
interchanging of a single, large heating block
with two smaller blocks. Some thermal cyclers
also allow the technician to control the
temperature of various areas of the block
separately.
14
To Order Find Out More Information On Thermal
Cycler Machines Please Come And Visit Us Today
Onwards..!
Gaia Science Pte Ltd Contact Us 102 E Pasir
Panjang Road, Singapore - 118529 Tel (65)
6276 8884 Fax [phone] Email
enquiry_at_gaiascience.com.sg Web
https//www.gaiascience.com.sg/