9 Tips to Optimize Antibody Your If Experiments

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9 Tips to Optimize Antibody Your If Experiments
antibody discovery
antibody humanization
single domain antibody
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1. Use the appropriate fixative for your sample
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2. Choose the right detergent to stain your
target.
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3. Use appropriate controls.
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4. optimize antibody dilution and cell density.
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5. Use blocking serum from a different organism
than the primary antibody.
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6. Primary and secondary antibody optimization.
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7. Multiple staining steps (optional).
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8. Employ counterstains to help identify cellular
landmarks.
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9. Get a proper sample mounting?