Priyanshi mishra (2)

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Dr. Harisingh gour central university sagar
(M.P)
Sieve
sampler (cascade sampler and Anderson cascade
sampler)
Submitted to- Dr. Yogesh Bhargav, (Department of
applied microbiology).
Submitted by- Priyanshi mishra Msc 2nd semester
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Sieve sampler

• Sieve sampler also known as sieve impaction
  sampler.
• Sieve samplers are the impaction devices, which
  use acceleration of air through numerous small
  hole (inlet orifices) in a perforated plate,
  which in turn impacts suspended particles in the
  air on surface of Agar.
• This is an aggressive and effective method of
  gathering air samples.

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Types of sieve sampler

1. Virtual sampler

2. Cascade sampler
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Cascade sampler

• A cascade impactor measures the reach range of a
  particulate substance as it moves through an
  opening with the use of aerosol.
• Used when particulate substance is moved through
  an opening with the use of aerosol.
• Is an measurement related device.
• Used to determine the particle size of
  distributed substance.
• Types of cascade sampler
• Automated cascade,
• Two stage impactor,
• Multi slit high volume cascade sampler,
• High volume cascade sampler,
• Anderson cascade sampler.

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Anderson cascade sampler

• The sampler was developed by Anderson in 1958.
• Anderson sampler is commonly used for indoor
  sampling.
• Is an ingenious device for selectively trapping
  different sizes of particles according to their
  size (momentum).

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Construction of Anderson cascade sampler

• This sampler consist of a stack of 6 metal
  section (6 to 8) that fit together to form an air
  tight cylinder.
• Each metal section has a perforated base. The
  number of perforation is same in each section
  (about 400). But the size of these perforation
  goes on decreasing as we move from upper section
  to lower section.
• Vacuum pump is connected to the air outlet so as
  to suck air.
• A flowmeter is provided to measure the volume of
  air passed through the sampler.
• An open Agar plate without lid is placed in each
  metal section (resting on three studs).
• A plate may contain one or more media.

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Construction of Anderson cascade sampler
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Working of Anderson cascade sampler

• When fully assembled the vacuum Pump is switched
  on. The air is sucked at the bottom of the unit,
  causing air to enter from the top and passes down
  through various section of the sampler.
• The air sucked at the top of the sampler travels
  at relatively low speed towards the first Agar
  plate, so only the larger particle (greater than
  7µm) impact onto the Agar surface and get
  trapped. The air then travels through the
  perforation to the second Agar plate, and so on
  till 5he last section of the sampler. As the same
  volume of air is forced to travel through
  successively smaller perforations, the air speed
  progressively increases. This raises the momentum
  of the air borne particles, so much that
  impaction of even very small particles (less than
  3µm diameter) is so strong against the Agar
  surface that they get trapped, particularly in
  the Agar plate keep in lower section.
• After the sampling has been run for 5-15 minutes
  or more, the vacuum pump is switched off and the
  metal section are separated. The petridishes are
  removed and incubated at suitable temperature for
  required period of time. The colonies may be
  bacteria, or fungi, which can then be
  identified.
• The colonies obtained on the plates in various
  sections gives valuable information regarding the
  size of particles present in the air and the
  chances of possible infection to various parts of
  respiratory tract.

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Working of Anderson cascade sampler
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Application of Anderson cascade sampler

• Ambient air studies,
• Indoor air studies,
• Virology investigations (including influenza and
  SARS),
• Pharmaceutical production,
• Filter and clean room efficiency studies,
• Composting,
• Wastewater treatment plants.

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Refrences

• http//www.theaudiopedia.com
• http//Aqicn.org
• http//Wwwwsciencedirect.com,
• http//www.Archive.bio.ed.ac.uk,
• http//www.rapidmicrobiology.com.

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??Thank you??