About Autoradiography Accessories

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ABOUT AUTORADIOGRAPHY ACCESSORIES
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ABOUT
Autoradiography is a well-known and sensitive
photochemical method used to record the spatial
distribution of radiolabeled compounds within an
object. Autoradiography accessories are also
used in the laboratory.
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TYPES

• Direct autoradiography
• In this autoradiography method, samples are
  placed with intimate contact with film produce
  black spots on the photographic film. Though this
  method is not used for high-energy particles such
  as ß-rays ?-rays as they pass through the film,
  they are detected by the indirect method.

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2. Indirect autoradiography In the Indirect
autoradiography technique, the emitted energy is
converted to light by using the scintillator or
fluorography. Scintillators are the molecules
that possess the capability to luminescence by
any ionizing radiation. So, every sample used in
this indirect method is coated with a
scintillator. Then the radioactive emissions
transfer their energy to the scintillator
molecules that produce photons they are exposed
to the photographic emulsion.
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APPLICATIONS

• Localization of a radioactive substance.
• Analyze the length number of DNA.
• PET SPECT for 3-dimensional localization of the
  radiation source.
• Quantitative analysis of macroautographs.

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