Plant Research Reagents

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Plant Research Reagents
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Introduction

• In the field of plant science, various studies
  such as the physiological actions, morphogenesis,
  and cultivation techniques of plants have been
  investigating.
• Tokyo Chemical Industry(TCI) offer a wide range
  of products used in plant research, such as
  plants clearing reagents and plant hormones.

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Plants Tissue-Clearing Reagent Suitable for
Fluorescent Protein Observation iTOMEI

• Improved TOMEI (iTOMEI) is a method that
  specializes TOMEI 1) for fluorescent protein
  observation by Prof. Sakamoto et al. This method
  enables simple transparency and allows for clear
  detection of fluorescent proteins.2,3,4)We offer
  suitable reagents for iTOMEI.
• Figure 1. Optical sections images of
  leaf of Arabidopsis thaliana obtained using
  confocal microscope. Figure 2.
  Cleared Arabidopsis thaliana by
  iTOMEI (left) Non-treatment, (right)
  iTOMEI treatment
• ProductsDecoloring solution T3940
  Tissue-Clearing Reagent iTOMEI-D for Plants
• Mounting solution T4003 Tissue-Clearing
  Reagent iTOMEI-M (RI 1.40) for Plants

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Advantages

• Enables transparency by simple osmosis in just a
  few days (more than 2 days).
• Keeps the fluorescence of a fluorescent protein
  such as GFP or tdTomato co-expressed as a
  reporter gene.
• Suppresses autofluorescence.
• Applicable to wide plant species including Oryza
  sativa, Arabidopsis thaliana, Marchantia
  polymorpha.

Video for how to use iTOMEI reagents
Please follow the link https//youtu.be/DM9IxPqh
pIs
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Rapid Transparency Reagent for Plants TOMEI

• TOMEI is clearing method suitable for observing
  only fluorescent staining dyes. It enables
  transparency in just a few hours (2 - 3
  hours).Product
• T3530 Tissue-Clearing Reagent TOMEI for
  Plants T3530 is unavailable except in
  Japan, U.S. and Europe

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Plant Growth Regulators

• A typical example of plant growth regulator is a
  plant hormone. Plant hormones are the collective
  term for compounds produced in minute amounts by
  plants to regulate their own physiological
  functions. To date, seven natural plant hormones
  have been discovered auxins, cytokinins,
  ethylene, jasmonates, abscisic acid, gibberillins
  and brassinosteroids (Figure 3).

Figure 3. Natural plant hormones
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Auxins

• Historically, auxins were first discovered as
  substances which showed phototropism. To date, it
  has been revealed that they play numerous roles
  such as initial development, budding, root growth
  development, growth of flower parts and cell
  division. Natural auxins are 3-indoleacetic acid,
  3-indolebutyric acid and phenylacetic acid. Some
  unnatural synthetic compounds also exhibit the
  same activities.

I0022 IAA
I0023 K-IAA
M2605 IAA Methyl Ester
E0878 IAA Ethyl Ester
I0024 IAN
I0026 IBA
I0032 IPA
N0005 NAA
N0007 Na-NAA
N0006 K-NAA
N0624 1-Naphthaleneacetamide
N0045 NOA
B2746 4-BPA
C0250 4-CPA
C0940 PCIB
C0206 MCPA
E1149 MCPB Ethyl Ester
D0396 2,4-D
D1319 Na-2,4-D Monohydrate
O0518 2,4-D Meptyl Ester
T1509 2,4,5-T Potassium Salt
T0451 TIBA
D1942 Dichlorprop
D4800 Dicamba
M3141 cvxIAA
A3390 5-Adamantyl-IAA
   
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Cytokinins

• Cytokinins are regarded as substances which
  stimulate cell division, shoot initiation and bud
  formation, when addition auxins are added.
  Typical structure features are adenine with an
  isopentenyl unit at N6 position, or with an
  isopentenyl unit with the methyl terminus being
  hydroxylated.

   
A0149 Adenine
A0151 Adenine Sulfate Dihydrate
B1088 N6-Benzyladenine
K0009 Kinetin
Z0012 trans-Zeatin
C0031 Carbanilide
R0079 Forchlorfenuron
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Abscisic Acids

• Abscisic acid is occasionally classified as a
  sesquiterpene, however, it is biosynthesized from
  a carotenoid (C40) precursor. It stimulates the
  closure of stomata in the absence of water and
  induces seeds to synthesize storage proteins. It
  is also released when a plant experiences stress,
  as in lack of nutrition, pests, root distress, or
  disease.
• A1698 (S)-()-Abscisic Acid

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Jasmonates

• Jasmomates have a distinct fragrance and are
  biosynthesized from linolenic acid, an
  unsaturated fatty acid. They inhibit growth in
  adverse conditions, and stimulate tuber
  formation. They promote senescence of leaves,
  suppression of fruit growth, and the induction of
  tuber formation in potatoes.

   
J0004 Jasmonic Acid (mixture of isomers)
M1068 Methyl Jasmonate (mixture of isomers)
D3225 Dihydrojasmonic Acid
D1431 Methyl Dihydrojasmonate (cis- and trans- mixture)
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Gibberellins
G0029 Gibberellin A3
Ethylene (Precursor)

• C1456 Ethephon