Glycan-related Reagents for Extracellular Vesicles (EVs) Research

1
Glycan-related Reagents for Extracellular Vesicle
(EV) Research
2
Extracellular Vesicles

• Extracellular Vesicles (EVs) are small vesicles
  with lipid bilayer that are secreted from various
  cells under physiological and pathological
  conditions and exist in the body fluids and cell
  culture supernatant.
• EVs contain nucleic acids and proteins, and act
  as mediators of intercellular communication by
  entering the target cells.
• EVs include subtypes such as exosomes and
  microvesicles.
• They are involved in many physiological responses
  and diseases, including cell differentiation,
  immune responses, infection, cancer,
  cardiovascular diseases, and neurodegeneration.

3
Extracellular Vesicles

• EVs derived from mesenchymal stem cells are known
  to have therapeutic potential, with several
  clinical trials ongoing.
• EV applications to drug delivery platforms have
  also been developed.

4
Glycans

• Glycans exist not only on the surface of cells,
  but also on the surface of sEVs (small
  Extracellular Vesicles EVs lt200 nm in diameter)
  as glycoproteins or glycolipids.
•  Importance of glycans on EVs has been shown. For
  example, glycocalyx on the cancer cell-derived
  EVs has been reported to involve in organotropic
  determination in cancer metastases.
• Glycans on the EV have been reported to play
  important role in the tuning of EV uptake.
• TCI has many products related to glycans
  expressed on the EVs.

5
Glycolipid-related Glycans

• Glycan patterns are well known to change during
  various biological events, which include cancer,
  inflammatory diseases, and immune diseases, and
  the same applies to sEV surface glycans.
• TCI can synthesize various glycans on the cell
  surface. We offers you oligosaccharides which
  play important roles such as intercellular
  recognition, adhesion, proliferation control and
  signaling.

6
Glycolipids

• Products

7
Glycolipids Products
G0509 a-GalactosylCeramide
G0580 Gb4 Ceramide
G0592 Gb5 Ceramide ( SSEA-3)
G0589 Globo-H Ceramide
G0593 SialylGb5 Ceramide ( SSEA-4)
G0483 Ganglioside GM1
G0489 Ganglioside GM3
G0422 Ganglioside GM3 (phyto-type)
G0510 Ganglioside GM3(Neu5Gc) (phyto-type)
S0910 Sialyl Neolactotetraosylceramide ( Sialyl nLc4Cer)
8
Oligosaccharides

• Products

9
Oligosaccharides Products
G0479 Globotriose
G0403 Gb3-ß-ethylazide
G0402 Gb3-ß-ethylamine
G0623 Gb4-PrN3
G0434 Globopentaose
G0599 Gb5-PrN3
G0596 Globo-H-PrN3
G0447 Globo-H-PrNH2
S0946 SSEA-1-PrNH2
S0849 Sialyl Lewis X-Lactose
N1147 Neu5Aca(2-6)Gal-ß-propylamine
N0950 Neu5Aca(2-6)Galß(1-4)GlcNAc-ß-ethylamine
N1249 Neu5Aca(2-3)Gal-ß-propylazide
N1146 Neu5Aca(2-3)Gal-ß-propylamine
N1267 Neu5Aca(2-3)Galß(1-4)GlcNAc-ß-propylamine
N1258 Neu5Aca(2-3)Galß(1-4)Glc-ß-propylamine
N1259 Neu5Gca(2-3)Galß(1-4)Glc-ß-propylamine
10
Anti-glycolipid Antibodies

• Antibodies are proteins which are one of the
  components of the immune system. The specificity
  of antibodies is likened to the interaction
  between a key and a keyhole. Anti-glycolipid
  antibodies can specifically recognize
  glycolipids.
• TCI mainly produce antibodies against
  glycolipids Ganglio-series, Globo-series,
  Lacto-series, and Neolacto-series.
• These antibodies can be used for
  immunohistochemistry, cell-staining, flow
  cytometry, ELISA, TLC-immunostaining and other
  methods.
• Our antibodies are very useful tools for
  analyzing the expression of carbohydrate chains
  and their functions.

11
Anti-glycolipid Antibodies

• Products

A2505 Anti-GM1 Monoclonal Antibody
A2576 Anti-GM2 Monoclonal Antibody
A2582 Anti-GM3 Monoclonal Antibody
A2507 Anti-GD1a Monoclonal Antibody
12
Anti-glycolipid Antibodies Products
A2508 Anti-GD1b Monoclonal Antibody
A3338 Anti-GD2 Monoclonal Antibody
A2580 Anti-GD3 Monoclonal Antibody
A2702 Anti-GT1a Monoclonal Antibody
A2732 Anti-GT1b Monoclonal Antibody
A2662 Anti-GQ1b Monoclonal Antibody
A2701 Anti-GalNAc-GD1a Monoclonal Antibody (2A3D2)
A2506 Anti-Gb3 Monoclonal Antibody
A2822 Anti-Gb3 Monoclonal Antibody Biotin Conjugate
A2706 Anti-SGPG (HNK-1) Monoclonal Antibody
13
Lectins

• Lectins are highly specific carbohydrate-binding
  proteins of nonimmune origin. Due to their
  ability to bind with cell-surface glycoproteins
  and glycolipids, lectins could agglutinate cells
  they also could reversibly associate
  polysaccharides and glycoproteins in solution.
• Lectin has long been well-known as tools for
  detection and analysis of functional
  oligosaccharides in the glycoscience field.
  Recombinant lectins show better stability
  compared with lectins extracted from natural
  resources.
• TCI offers not only recombinant lectins but also
  chemically modified lectins such as several types
  of biotinylated lectin and lectin-agarose for
  detection and capturing of glycoconjugates.

14
LecBeads (Lectin-Agarose)

• Products

R0235 rPSL1a-LecBeads for Siaa(2-6)Gal
R0236 rLSL-N-LecBeads for Galß(1-4)GlcNAc, poly LacNAc
R0237 rMOA-LecBeads for Gala(1-3)Gal
R0238 rSRL-LecBeads for GlcNAcß(1-2)Man, Galß(1-3)GalNAc
R0239 rGRFT-LecBeads for Mana(1-2)Man
A3331 AOL-LecBeads for Fuca(1-6)/a(1-4)/a(1-3)/a(1-2)
15
Lectin-Biotin Conjugates

• Products

R0230 rPSL1a-Biotin for Siaa(2-6)Gal
R0231 rLSL-N-Biotin for Galß(1-4)GlcNAc, poly LacNAc
R0232 rMOA-Biotin for Gala(1-3)Gal
R0233 rSRL-Biotin for GlcNAcß(1-2)Man, Galß(1-3)GalNAc
R0234 rGRFT-Biotin for Mana(1-2)Man
A2659 AOL-Biotin Conjugate
16
Lectins

• Products

R0225 Recombinant Polyporus squamosus lectin ( rPSL1a) expressed in E. coli
R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain ( rLSL-N) expressed in E. coli
R0227 Recombinant Marasmius oreades agglutinin ( rMOA) expressed in E. coli
R0228 Recombinant Sclerotium rolfsii lectin ( rSRL) expressed in E. coli
R0229 Recombinant Griffithsia sp. lectin ( rGRFT) expressed in E. coli
L0169 Lectin, Fucose specific ( AOL) from Aspergillus oryzae
17
Endoglycosidases

• Endoglycoceramidase (EGCase) is a
  glycolipid-specific hydrolase that cleaves the
  glycosidic linkage between oligosaccharide and
  ceramide of various glycosphingolipids (GSLs).
• Recombinant EGCase (rEGCase) is activated under a
  detergent-dependent condition. In contrast,
  Activator II is capable of inducing activity of
  EGCases without any detergent reagents.
• By the use of Activator II, GSLs on cell surfaces
  of living cells could be hydrolyzed without cell
  disruption caused by detergent.

18
Endoglycosidases and Related Enzymes

• Products

R0240 rEGCase I
R0241 rEGCase I assisted by Activator II
R0242 rEGCase II
R0243 rEGCase II assisted by Activator II
A1651 Endo-M ( endo-ß-N-Acetylglucosaminidase)
G0365 Glycosynthase ( Endo-M-N175Q)
E1339 Endo-M-W251N
A1844 endo-a-N-Acetylgalactosaminidase (Endo-a)
K0069 Keratanase II