Chromatography: Plate concept

1
ChromatographyPlate concept

• A chromatographic column is assumed to be made up
  of a large number of hypothetical plates
• Each of these plates is equivalent to one
  equilibrium stage
• The plate concept is borrowed from distillation
• The greater the number of plates, the better the
  separation is likely to be
• The height equivalent to a theoretical plate
  (HETP or simply H) should be as small as possible

2
van Deemter Equation
opt
opt
u
3
Resolution
Shape and yield of peak
4
Gas chromatography
Gas-solid chromatography Gas-liquid
chromatography
Gas chromatography detectors TCD, FID and ECD