Cell line Master Culture PowerPoint PPT Presentation

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Title: Cell line Master Culture


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Cell line Master Culture
Bulk Cell culture production (adherent or
suspension cultures)
Conditioned Media (from adherent or suspension
cultures)
Mammalian Protein Expression (Transient or
stable )
Bacterial Protein Expression
DNA Sequencing-DNA Synthesis
Plasmid Preparation
Clone Identification
Transient and Stable Expression in Mammalian Cells
Bacterial/ Cell /Tissue Lysate Preparation
Cellular Protein Characterization
Cellular Protein/organelles Preparation
Affinity Media Generation
SDS-PAGE, Urea or IEF-PAGE
Western and Dot blotting
Lyophilization
Protein-protein interactions
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