Oregon Wolfe Barley Map

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• Oregon Wolfe Barley Map
• 187 classical loci

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Locus abbreviations and alleles Vrs1 V V,
v GBSS W W,w Nud N N,n LKs2 L L,l
OWB D 2-row, normal starch, hulled, long awn
Vrs1Vrs1, GBSS-1GBSS, NudNud, LKs2Lks2 VVWWNNLL
_V______ _W_________N__L_______ _V______
_W_________N__L_______
OWB R 6-row, waxy starch, hull-less, short
awn vrs1vrs1,gbss-1gbss-1,nudnud,lks2lks2 vvwwnnll
_v______ _w_________n__l_______ _v______
_w_________n__l_______
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Goal 2 row, waxy, hulled, short awn VVwwNNll
OWB R 6-row, waxy starch, hull-less, short
awn vvwwnnll
OWB D 2-row, normal starch, hulled, long awn
VVWWNNLL
X
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F1
_V______ _W_________N__L_______ _v______
_w__________n__l_______
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Pachynema in meiosis in F1 plant showing paired
homologous chromosomes (bivalents)
_V______ _W_________N__L_______ _V______
_W_________N__L_______ _v______ _w__________n__
l_______ _v______ _w__________n__l_______
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Pachynema in meiosis in F1 plant showing paired
homologous chromosomes (bivalents)
_V______ _W__________________ _V______
_W__________________ _v______ _w_______________
____ _v______ _w___________________
Gametes VW VW vw vw
DH plants VVWW VVWW vvww vvww
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Pachynema in meiosis in F1 plant showing paired
homologous chromosomes (bivalents)
_V______ _w__________________ _V______
_w__________________ _v______ _W_______________
____ _v______ _W___________________
DH plants VVww VVww vvWW vvWW
Gametes Vw Vw vW vW
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Goal 2 row, waxy, hulled, short awn VVwwNNll
Independent assortment due to random alignment of
non-homologous chromosomes at Metaphase I - VVww
parentals non-parentals independent
assortment
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Pachynema in meiosis in F1 plant showing paired
homologous chromosomes (bivalents)
_V______ _W_________N__L_______ _V______
_W_________N__L_______ _v______ _w__________n__
l_______ _v______ _w__________n__l_______
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Pachynema in meiosis in F1 plant showing paired
homologous chromosomes (bivalents)
_V______ _W_________N__L_______ _V______
_W_________N__L_______ _v______ _w__________n__
l_______ _v______ _w__________n__l_______
X
DH plants VVWWNNLL VVWWnnll vvwwNNLL vvwwnnll
Gametes VWNL VWnl vwNL vwnl
Goal 2 row, waxy, hulled, short awn VVwwNNll
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Pachynema in meiosis in F1 plant showing paired
homologous chromosomes (bivalents)
_V______ _w_________n__l_______ _V______
_w_________n__l_______ _v______ _W_________N__L
______ _v______ _W_________ N__L______
X
DH plants VVwwnnll VVwwNNLL vvWWnnll vvWWNNLL
Gametes Vwnl VwNL vWnl vWNL
Goal 2 row, waxy, hulled, short awn VVwwNNll
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Pachynema in meiosis in F1 plant showing paired
homolowous chromosomes (bivalents)
_V______ _w_________n__l_______ _V______
_w_________n__l_______ _v______ _W_________N__L
______ _v______ _W_________ N__L______
X
DH plants VVwwNNLL VVwwnnll vvWWnnll vvWWNNLL
Gametes VwNL Vwnl vWnl vWNL
Goal 2 row, waxy, hulled, short awn VVwwNNll
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Pachynema in meiosis in F1 plant showing paired
homologous chromosomes (bivalents)
_V______ _w_________n__l_______ _V______
_w_________n__l_______ _v______ _W_________N__L
______ _v______ _W_________ N__L______
X
DH plants VVwwnnll VVwwNNLL vvWWNNLL vvWWnnll
Gametes Vwnl VwNL vWNL vWnl
Goal 2 row, waxy, hulled, short awn VVwwNNll
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Goal 2 row, waxy, hulled, short awn VVwwNNll
Independent assortment due to crossovers between
loci on same chromosome - wwNN
parentals non-parentals independent
assortment
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Pachynema in meiosis in F1 plant showing paired
homologous chromosomes (bivalents)
_V______ _W_________N__L_______ _V______
_W_________N__L_______ _v______ _w__________n__
l_______ _v______ _w__________n__l_______
X
DH plants VVWWNNLL VVWWNNll vvwwnnLL vvwwnnll
Gametes VWNL VWNl vwnL vwnl
Goal 2 row, waxy, hulled, short awn VVwwNNll
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Parentals gt non-parentals linkage Linkage is
calculated as the total non-parental types/ total
population size 12/94 12.7 recombination (13.1
Kosambi cM 14.7 Haldane cM)
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Pachynema in meiosis in F1 plant showing paired
homologous chromosomes (bivalents)
_V______ _w_________n__ l_______ _V______
_w_________n__ l_______ _v______ _W_________N__
L______ _v______ _W_________ N__L______
X
X
DH plants VVwwnnll VVwwNNll vvWWnnLL vvWWNNLL
Gametes Vwnl VwNl vWnL vWNL
Goal 2 row, waxy, hulled, short awn VVwwNNll
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Goal 2 row, waxy, hulled, short awn VVwwNNll
Independent assortment due to crossovers between
loci on same chromosome - wwNN
The Chi-square test for linkage is Prob( X2 gt
7.86 Degrees of Freedom3 ) lt 0.048995 Accept
or reject? Evidence for very weak linkage see
lab example
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Haldane Distance
r0.36 0.32 0.34
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Kosambi Distance
r0.36 0.32 0.34
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Mapping Functions
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• Oregon Wolfe Barley Map
• 187 classical loci

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Oregon Wolfe Barley Linkage Map (2383 loci,
Haldane cM)
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