WSU Central Instrument Facility

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WSU Central Instrument Facility

• Prof. David M. Coleman
• WSU Chemistry CIF Director
• Professional Academic Development Seminar on
  Core Research Facilities at WSU
• 23 October, 2009

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WSU Chemistry Department
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Wayne State University
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WSU Central Instrument Facility

• Overview
• a.) What is a core facility and who does it
  support?
• b.) Shared instruments vs. individually-run
  instruments.
• c.) Quick virtual tour today.

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WSU Central Instrument Facility

• Issues
• a.) Valuation 15M
• b.) Staffing Ph.D.-level Managers of
• each Department (7 Total)
• c.) Users (Internal External)
• d.) Funding Ongoing User Fees
• e.) Funding New Instruments (NSF NIH)
  Donations.
• f.) Service Maintenance
• g.) Oversight Committee
• h.) 7,000 Square Feet
• i.) Faculty Recruiting, Grant intake
• to University, Encourage
• Collaboration through High Levels
• of Technical Expertise

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Central Instrumentation Facility
(Available Services)
III. X-RAY
I. NMR
Dr. Mary Jane Heeg
Dr. Bashar Ksebati
II. Mass Spectrometry
Dr. Brian J. Shay Dr. Lew Hryhorczuk
V. TEM/SEM
Dr. Sam Shinozaki
VI. Glassblowing Services
IV. Computer Support
Marty A. Krol Nestor Ocampo
David G. Daenzer
For further information contact Prof. David M.
Coleman, Director - dmc_at_wayne.chem.edu
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WSU Central Instrument Facility

• Areas
• a.) X-Ray
• b.) NMR
• c.) Mass Spectrometry
• d.) Electron Microscopy
• e.) Small Instruments / Spectroscopy
• f.) Glassblowing Services
• g.) Computer/Electronic Support

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WSU Central Instrument Facility
Mission Statement The mission of the CIF is
to provide friendly, professional, and timely
high-quality research and teaching support
services to faculty, graduate, and undergraduate
students of Wayne State University and the
Chemistry Department. The CIF will also provide
such services to the greater Detroit-area and
Michigan scientific community.
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WSU Central Instrument Facility XRay
Crystallography

Manager Dr. Mary Jane Heeg (A.) A single
crystal Bruker APEXII/Kappa diffractometer.
State of the art instrument with graphite
detector and APEX-2 detector. Routinely used at
100K at sample site. Full structural details
from crystals 0.2 mm to 0.002 mm. (B.) Rigaku
D-MAX/B powder diffractometer for structural
determinations of polycrystalline materials.
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WSU Central Instrument Facility - XRAY

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WSU Central Instrument Facility - XRAY

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WSU Central Instrument Facility - Microscopy /
TEM SEM

Managers Drs. Brian Shay Lew Hryhorczuk
1.) Micromass Quatro LC Triple-Quad (uses
electrospray sample ionization for fragile
(biological) molecules 2.) Bruker Daltronics
TOF-300 MALDI Ultraflex (allows for vaporization
and ionization of non-volatile biological and
other samples using a time-of-flight mass
spectrometer (TOF-MS) 3.) Micromass VA-GCT
Orthogonal TOF Mass Spectrometer for exact mass
determination for synthetic chemists. CG and
direct probe sample introduction (w/ EI, CI, DCI,
FI, etc. ionization) 4.) Micromass ZQ2000-LC
with autosampler/PDA/Electrospray/Single-Quad for
sample screening 
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WSU Central Instrument Facility - Microscopy /
TEM SEM

Managers Drs. Brian Shay Lew Hryhorczuk
5.) Hewlett-Packard 5988A quadrupole CG/MS
for routine low-resolution experiments. 6.)
Waters LCT Premier time of flight mass
spectrometer equipped with electrospray
ionization which is used for determination of
high resolution mass spectrometric  data of a
wide variety of samples, but especially for
oligosaccharides and glycopeptides. 7.)
Proposal pending (NIH) for a MALDI TOF-TOF
instrument. 8.) Proposal pending (NIH) for a
replacement Triple-Quad instrument.
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Waters GCT TOF
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Quattro LC
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ESI Mechanism of Ion Formation
Ions are ejected when coulomb repulsion in the
droplet exceeds the surface tension.
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Bruker Daltonics ultraflexTM MALDI TOF
ultraflexTM
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WSU Central Instrument Facility - NMR

Manager Dr. Bashar Ksebati 1.) Varian Gemini
300 (300 MHz-proton/carbon/ capable of advanced
experiments such as APT, coSY, NOESY, etc.)
2.) Varian Mercury-400 (400 MHz-multinuclear,
with variable temperature and pulsed field
gradients/ tuned for observing 1H, 19F, 31P and
13C) 3.) Varian VNMRS-400 (400
MHz-multinuclear, with variable temperature and
pulsed field gradients/ tuned for observing 1H,
19F, 31P and 13C). Installed new in 2009 4.)
Varian VNMRS-500 (500 MHzmultinuclear, variable
temperature instrument especially useful for
study of small sample amounts or complex
biomolecules note new console and probes were
installed in 2007)
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WSU Central Instrument Facility - NMR

Manager Dr. Bashar Ksebati 5.) A new 600 MHz.
NMR was funded in Sept., 2009. Selections
currently being made. 6.) Bruker 700 MHz with
liquid helium cryoprobe multinuclear, variable
temperature instrument for the study of complex
biomolecules. A quadruple-resonance probe is
capable of synthesis/detection of 1H, 13C, 15N,
and 31P r.f. signals and XYZ gradients. 7.) A
Bruker EPR is also included with room temperature
and two cryoprobes.
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WSU Central Instrument Facility - NMR - 700 MHz

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WSU Central Instrument Facility - Microscopy /
TEM SEM

Manager Dr. Yi Liu 1.) a 200kV JEOL FASTEM
2010 Transmission Electron Microscope includes
numerous sample preparation accessories and
holders and an energy dispersive spectrometer
(EDAX) for examination of materials,
nanoparticles, etc. 2.) A Hitachi S-2400 SEM
Scanning Electron Microscope with EDAX analytical
capability. Electron Beam Lithography
capabilities were recently added to the SEM with
internal funding. 3.) A State of the Art FE
SEM recently funded by NSF. Will have many
capabilities including biochemical applications.
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WSU Central Instrument Facility - TEM

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WSU Central Instrument Facility - TEM

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WSU Central Instrument Facility - Misc.
Instruments

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WSU Central Instrument Facility

• Prof. David M. Coleman
• WSU Chemistry Associate Chair CIF Director
• WSU Biology Dept. Colloquium
• 14 Nov., 2005

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Small Shared Instruments (partial list)
  Varian/Digilab Excalibur 3100 High Resolution
FT-IR with NIR, Far-IR, and Microscope
capability. Varian/Digilab Victory 2200
FT-IR JASCO FP-6500 Spectrofluorometer JASCO
V-570 UV/VIS/NIR Spectrometer w/ intergrating
sphere Applied Photophysics Chirascan high
performance circular dichroism spectrometer with
accessories. Perkin-Elmer automated
polarimeter Perkin-Elmer Atomic Absorption
Spectrometer (flame and graphite
furnace) Isothermal calorimeter Numerous other
instruments for teaching and research

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CIF Fee Structure -

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WSU Central Instrument Facility -

1.) National Science Foundation
(NIH) 2.) National Institutes for Health
(NIH) 3.) Wayne State University 4.) Lumigen 5.
) Ford Motor Company 6.) Michigan Life Sciences
Corridor 7.) Our Customers
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Contact Information
1.) David M. Coleman dmc_at_chem.wayne.edu 2.) Open
24/7 for all trained users. Card
Access. 3.) Resource Manager 4.) Competitive
Fee Structure. ALL WSU users pay the same
rate. 5.) chem.wayne.edy/facilities/index/html 6
.) Happy to arrange for a tour/visit 7.) Happy
to establish new users, research collaborations,
etc.