BIO488L: Medical Microbiology Lab Exercise 3

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BIO488L Medical Microbiology Lab Exercise 3

• Spring 2006
• T.A. Sami Kemper

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Today

• Quiz 1
• Expectations
• Enterobacteriaceae Unknown Culture
• YOU WILL NEED TO CHECK THESE AT 24 HOURS!!!!!!!
• Clinical Unknown Culture

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Enterobacteriaceae Unknown

• Record the letter of your unknown
• Innoculate your enterotube
• Check your results at 24 hours
• Paper on the unknown is due in one week.
• Rubric
• Only one submission

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Enterotube II

• Combines multiple biochemical tests
• Tests
• Glucose fermentation (acid or acid and gas)
• Lysine decarboxylase
• Ornithine decarboxylase

• Hydrogen sulfide production
• Indole production
• Adonitol, lactose, sorbitol and dulcitol
  fermentation
• Voges-Proskauer
• Phenylalanine deaminase
• Urase
• Citrate utilization

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Innoculation of the Enterotube
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Reading the Enterotube
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Clinical Unknown

• 2 organisms
• Both must be accurately identified
• You are responsible for determining what tests
  must be done and requesting them
• Paper is due on the last day of wet lab (before
  presentations start)
• Rubric
• Only one submission

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Clinical Unknown Hints

• Keep the culture pure!
• Gram stain- with positivea nd negative controls
  on the other side of the slide
• Think carefully about what tests you need to do
  and request them early
• Take good notes