Indirect ? Use of barley and wheat SSR primer sets

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EXPANSION OF OAT-BASED MICROSATELLITE RESOURCES
USING BOTH DIRECT AND INDIRECT STRATEGIES Eric
Jackson, J. Anderson, M. Carson, D. Obert, G. Hu,
and M. Bonman
USDA ARS Aberdeen, Molecular Genetics Laboratory
Robert Campbell Biological Technician
Irene Shackelford Biological Technician
Indirect ? Use of barley and wheat SSR primer
sets 146 barley 210 wheat Direct ?
Development of oat-based SSR enriched
libraries AC ATT ATG CATC
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Polymorphic 3/6 3/6
3/6 5/6 4/6 3/6 3/6
3/6 3/6 2/6 3/6 2/6
TAM O-405
TAM O-301
MN-Chong
MN841810
Ogle1040
A.m.-dom
MN-Rines
Ogle157
A. m-wt
Noble-2
Makuru
Kanota
Otana
Stout
SunII
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SSR Markers
Co-dominant SSR
Dominant SSR
Dominant deg-SSR
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Polymorphic markers AB_AM_17 Dom. AB_AM_20
Dom. AB_AB_28 Co-dom. AB_AM_29 Dom. AB_AM_31
Dom. AB_AM_32 Dom. AB_AM_35
Co-dom. AB_AM_53 Dom.
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CONCULSIONS 31 that amplified between Ogle and
TAM O-301 contained degenerative SSR motifs,
while one complete SSR motif was
identified. 78.6 sequenced from the AG- and
ATG- libraries contained SSR motifs for which
working primers could be designed. 25.5 of the
markers were polymorphic between Ogle and TAM
O-301 (agarose) and 43 of those were
polymorphic. Sequencing of the two libraries
has yielded approximately 1700 candidate SSR
primer sets. ? 1336
working primers ?
300 polymorphic in OT
? 130 co-dominant